10.5281/zenodo.45833
Zattara, Eduardo E
Eduardo E
Zattara
University of Maryland, College Park
Turlington, Kate W
Kate W
Turlington
University of Maryland, College Park
Bely, Alexandra E
Alexandra E
Bely
University of Maryland, College Park
Long-Term Time-Lapse Live Imaging Reveals Extensive Cell Migration During Annelid Regeneration
Zenodo
2016
annelid neoblast
cell migration
developmental dynamics
growth rates
in-vivo studies
regeneration
time-lapse imaging
2016-02-09
https://zenodo.org/record/45833
Creative Commons Attribution-NonCommercial 4.0
Open Access
<p>Supporting Materials for the article:<br />
Long-term time-lapse live imaging reveals extensive cell migration during annelid regeneration</p>
<p>Eduardo E. Zattara, Kate W. Turlington and Alexandra E. Bely</p>
<p>BMC Developmental Biology, 2016</p>
<p>Includes 11 movies and one compressed file with R code and data tables.</p>
Here we describe a new method for long-duration time-lapse imaging of adults of the small freshwater annelid Pristina leidyi and use this method to investigate its regenerative processes. Specimens are immobilized with tetrodotoxin, resulting in irreversible paralysis yet apparently normal regeneration, and mounted in agarose surrounded by culture water or halocarbon oil, to prevent dehydration but allowing gas exchange. Using this method, worms can be imaged continuously and at high spatial-temporal resolution for up to 5 days, spanning the entire regeneration process. We performed a fine-scale analysis of regeneration growth rate and characterized cell migration dynamics during early regeneration. Our studies reveal the migration of several putative cell types, including one strongly resembling published descriptions of annelid neoblasts, a cell type suggested to be migratory based on "still-shot" studies and long hypothesized to be linked to regenerative success in annelids.