10.5068/D14370
Boocock, James
0000-0003-0323-8818
University of California Los Angeles
Sadhu, Meru
Genetic Disease Research Branch, National Human Genome Research Institute
Bloom, Joshua
University of California Los Angeles
Durvasula, Arun
University of California Los Angeles
Kruglyak, Leonid
University of California Los Angeles
Ancient balancing selection maintains incompatible versions of the
galactose pathway in yeast (Figure creation)
Dryad
dataset
2020
2020-10-26T00:00:00Z
2020-10-26T00:00:00Z
en
5963731162 bytes
3
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Metabolic pathways differ between species, but are expected to be similar
within a species. We discovered two functional, incompatible versions of
the galactose pathway in S. cerevisiae. We identified a 3-locus genetic
interaction for growth in galactose, and used precisely engineered alleles
to show that is arises from variation in the metabolic genes GAL2,
GAL1/10/7, and PGM1, and that the reference allele of PGM1 is incompatible
with the alternative alleles of the other genes. Multi-locus balancing
selection has maintained the two incompatible versions of the pathway for
millions of years. Strains with alternative alleles are found primarily in
galactose-rich dairy environments, and they grow faster in galactose, but
slower in glucose, revealing a tradeoff on which balancing selection may
have acted.
This dataset contains all the files necessary to recreate the figures in
Boocock et al "Ancient balancing selection maintains incompatible
versions of the galactose pathway in yeast".