10.5061/DRYAD.ZW3R2285N
Crain, Jared
0000-0001-9484-8325
Kansas State University
DeHaan, Lee
The Land Institute
Poland, Jesse
Kansas State University
Genomic prediction enables rapid selection of high-performing genets in an
intermediate wheatgrass (Thinopyrum intermedium) breeding program
Dryad
dataset
2020
Thinopyrum intermedium
Genomic selection
perennial grains
breeding cycle
realized genomic selection performance
expected genomic selection performance
Malone Family Foundation
https://ror.org/05mmamd82
2021-12-04T00:00:00Z
2021-03-04T00:00:00Z
en
https://doi.org/10.1002/tpg2.20080
2504808389 bytes
12
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
In an era of constrained and depleted natural resources, perennial grains
could provide sustainable food production in addition to beneficial
ecosystem services like reduced erosion and increased atmospheric carbon
capture. One such perennial, intermediate wheatgrass (IWG; Thinopyrum
intermedium) has been undergoing continuous breeding for domestication and
improvement to develop a perennial grain crop since the 1980’s. However,
as a perennial species, IWG has required 2-5 years per selection
generation. Therefore, starting in 2017 genomic selection was initiated in
the breeding program at The Land Institute, Salina, KS, enabling one
complete cycle per year. For each cycle, ~4,000 seedlings were profiled
using genotyping-by-sequencing and genomic estimated breeding values were
calculated. Selection identified 100 individuals to advance as parents
each generation, while validation populations for model updating
consisting of 1,000-1,200 were also selected. The crossing block
populations were randomly intermated in a greenhouse to form the
subsequent cycle, while the validation populations were transplanted to
irrigated and non-irrigated field sites for phenotypic evaluations in the
following years. For priority breeding traits of seed mass, free
threshing, and non-shattering, correlations between predicted values and
observed data were greater than 0.51 and all traits ranged from 0.02-0.76.
The realized selection differential ranged from 19-23% for selected
traits, and the expected genetic gains for these traits, including spike
yield, ranged from 5-15% per year. Genomic selection is a powerful tool to
speed the domestication and development of intermediate wheatgrass and
likely other perennial crops with extended breeding timelines.
See manuscript and readme file.
Read me file documents data and process.