10.5061/DRYAD.ZKH18935N
Martinez, Adam
0000-0002-8627-4306
Johannes Gutenberg University of Mainz
Kaltenpoth, Martin
0000-0001-9450-0345
Johannes Gutenberg University of Mainz
Sandoval-Calderón, Mario
Johannes Gutenberg University of Mainz
Ingham, Chantal
Johannes Gutenberg University of Mainz
Deckert, Juergen
0000-0003-4211-4463
Museum für Naturkunde
Salem, Hassan
Prirodnjacki Muzej Crne Gore
Onchuru, Thomas
Johannes Gutenberg University of Mainz
Angiosperm to Gymnosperm host-plant switch entails shifts in microbiota of
the Welwitschia bug, Probergrothius angolensis (Distant, 1902)
Dryad
dataset
2019
Deutsche Forschungsgemeinschaft
https://ror.org/018mejw64
MA7282/1-1
2019-10-25T00:00:00Z
2019-10-25T00:00:00Z
en
1183128 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Adaptation of herbivorous insects to new host plants is key to their
evolutionary success in diverse environments. Many insects are associated
with mutualistic gut bacteria that contribute to the host’s nutrition and
can thereby facilitate dietary switching in polyphagous insects. However,
how gut microbial communities differ between populations of the same
species that feed on different host plants remains poorly understood. Most
species of Pyrrhocoridae (Hemiptera: Heteroptera) are specialist
seed-feeders on plants in the family Malvaceae, however populations of one
species, Probergrothius angolensis, has switched to the very distantly
related Welwitschia mirabilis plant in the Namib Desert. We first compared
development and survival of laboratory populations of Pr. angolensis with
two other pyrrhocorids on seeds of Welwitschia and found only Pr.
angolensis capable of successfully completing its development. We then
collected Pr. angolensis in Namibia from Malvaceae and Welwitschia host
plants, respectively, to assess their bacterial and fungal community
profiles using high-throughput amplicon sequencing. Comparison with
long-term lab reared insects indicated stable associations of Pr.
angolensis with core bacteria (Commensalibacter, Enterococcus, Bartonella,
and Klebsiella), but not fungi or yeasts. Phylogenetic analyses of core
bacteria revealed relationships to other insect-associated bacteria, but
also found new taxa indicating potential host-specialized nutritional
roles. Importantly, the microbial community profiles of bugs feeding on
Welwitschia vs. Malvaceae revealed stark and consistent differences in the
relative abundance of core bacterial taxa that correlate with the
host-plant switch; a result we were able to recreate through feeding
experiments. Thus, a dynamic gut microbiota may provide a means for insect
adaptation to new host plants in new environments when food plants are
extremely divergent.
Quantitative PCR (qPCR) was performed on a RotorGene Q seed_qPCR.xlsx is
qPCR performed on the insect seed diet
qPCR_Pangolensis_DifferentDiets.xlsx is the qPCR performed on insects fed
the different seed diets. Probergrothius angolensis gene alignments
(MUSCLE) (untrimmed/unmasked) .phy files Genes: (Genbank MN542943
to MN543046) SCR Mitochondrial 16S ITS2 ITS1 dfd2 CytB CO1 CO2 abdA 18S
Illumina MiSeq V3 Chemistry (Primers 515F-Y and 926R)
16S_AmpliconSequenceVariants_LabColony.xlsx is the ASV table for the
analysis of the lab colony of P. angolensis. Qiime2_Analysis_Pipeline.txt
is the analysis pipeline for the 62 P. angolensis samples from Namibia.
negC-V4V5_S67.report.pdf is the report for the MiSeq negative control.
16S_AmpliconSequenceVariants_Namibia.xlsx is the ASV table for the
analysis of the P. angolensis collected in Namibia. Firebug development
assay Firebug_development_on_different_seeds.xlsx is the table containing
survival, development, and fresh weight info for bugs fed the different
seed diets.