10.5061/DRYAD.X69P8CZFB
Seear, Paul
0000-0003-4303-5943
University of Leicester
France, Martin
University of Leicester
Gregory, Catherine
University of Leicester
Heavens, Darren
Earlham Institute
Schmickl, Roswitha
Charles University
Yant, Levi
University of Nottingham
Higgins, James
University of Leicester
Nucleotide alignments of eight meiosis genes under extreme selection
following whole genome duplication in Arabidopsis lyrata/A.arenosa.
Dryad
dataset
2020
European Research Council
https://ror.org/0472cxd90
BB/M01973X/1 and BB/P013511/1
European Research Council
https://ror.org/0472cxd90
ERC-StG 679056 HOTSPOT
2020-06-24T00:00:00Z
2020-06-24T00:00:00Z
en
https://doi.org/10.1371/journal.pgen.1008900
5310221 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
In this study we performed a genotype-phenotype association analysis of
meiotic stability in 10 autotetraploid Arabidopsis
lyrata and A. lyrata/A. arenosa hybrid populations collected from the
Wachau region and East Austrian Forealps. The aim was to determine the
effect of eight meiosis genes under extreme selection upon adaptation to
whole genome duplication. Individual plants were genotyped by
high-throughput sequencing of the eight meiosis genes
(ASY1, ASY3, PDS5b, PRD3, REC8, SMC3, ZYP1a/b) implicated in synaptonemal
complex formation and phenotyped by assessing meiotic metaphase I
chromosome configurations. Our results reveal that meiotic stability
varied greatly (20–100%) between individual tetraploid plants and
associated with segregation of a novel ASYNAPSIS3 (ASY3) allele derived
from A. lyrata. The ASY3 allele that associates with meiotic stability
possesses a putative in-frame tandem duplication (TD) of a serine-rich
region upstream of the coiled-coil domain that appears to have arisen at
sites of DNA microhomology. The frequency of multivalents observed in
plants homozygous for the ASY3 TD haplotype was significantly lower than
in plants heterozygous for ASY3 TD/ND (non-duplicated) haplotypes. The
chiasma distribution was significantly altered in the stable plants
compared to the unstable plants with a shift from proximal and
interstitial to predominantly distal locations. The number of HEI10 foci
at pachytene that mark class I crossovers was significantly reduced in a
plant homozygous for ASY3 TD compared to a plant heterozygous for ASY3
ND/TD. Fifty-eight alleles of the 8 meiosis genes were identified from the
10 populations analysed, demonstrating dynamic population variability at
these loci. Widespread chimerism between alleles originating
from A. lyrata/A. arenosa and diploid/tetraploids indicates that this
group of rapidly evolving genes may provide precise adaptive control over
meiotic recombination in the tetraploids, the very process that gave rise
to them.