10.5061/DRYAD.X0K6DJHJ4
Kinser, Taliesin
0000-0002-9497-5399
University of Florida
Smith, Ronald
William & Mary
Lawrence, Amelia
William & Mary
Cooley, Arielle
0000-0003-3730-0288
Whitman College
Vallejo-Marín, Mario
0000-0002-5663-8025
University of Stirling
Conradi Smith, Gregory
William & Mary
Puzey, Joshua
William & Mary
Endosperm-based incompatibilities in hybrid monkeyflowers
Dryad
dataset
2021
FOS: Biological sciences
Genomic imprinting
Endosperm
Mimulus
Epigenetics
Histology
bisulfite sequencing
Subgenome Dominance
The College of William and Mary Research Award*
The College of William and Mary Research Award
2021-04-06T00:00:00Z
2021-04-06T00:00:00Z
en
2109853198 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The sexual endosperm is an angiosperm innovation central to flowering
plant reproduction. Genomic interactions between parental alleles control
its development and help determine seed viability. These interactions are
characterized by genomic imprinting, where expression from certain genes
is parent-specific. Unsuccessful imprinting has been linked to failed
hybridization between plants of different species or ploidies. Here, we
describe an endosperm-based barrier between Mimulus guttatus, a diploid,
and M. luteus, an allotetraploid. Hybrid seeds suffer from underdeveloped
endosperm, reducing viability, when M. guttatus is the seed parent, and
from arrested endosperm and abortion when M. luteus is the seed parent.
The two parental species differ in patterns of endosperm DNA methylation,
expression dynamics, and their sets of imprinted genes; and transgressive
patterns of methylation and expression emerge in their hybrids. The two
inherited M. luteus subgenomes, which are genetically distinct but
epigenetically similar, are expressionally dominant over the M. guttatus
genome in the hybrid embryo and especially endosperm, where paternal
imprints, in particular M. luteus', are perturbed. We suggest that
diverged epigenetic landscapes between parental genomes result in
epigenetic repatterning in hybrids that drive global shifts in expression
patterns and can lead to endosperm-based hybridization barriers.
All data herein was derived from seeds produced from intraspecific or
interspecific crosses among lines of Mimulus guttatus and M. luteus. All
plants were stored and all crosses performed at the College of William and
Mary in either growth chambers or a greenhouse under standardized growth
conditions. Please refer to the ReadMe files in each directory
(Cross_section_images, Expression_data, Methylation data, Morphology_data)
and the 'Methods' section in the associated manuscript for
further details regarding data collection and processing.
For details on file contents, please refer to the ReadMe files in each
directory (Cross_section_images, Expression_data, Methylation data,
Morphology_data). Raw sequence data is available on NCBI's Sequence
Read Archive (SRA# = SUB9317876).