10.5061/DRYAD.VH396
Cooper, Brandon S.
University of California, Davis
Ginsberg, Paul S.
University of California, Davis
Turelli, Michael
University of California, Davis
Matute, Daniel R.
University of North Carolina
Data from: Wolbachia in the Drosophila yakuba complex: pervasive frequency
variation and weak cytoplasmic incompatibility, but no apparent effect on
reproductive isolation
Dryad
dataset
2016
Drosophila santomea
frequency variation
Wolbachia
Drosophila teissieri
host-microbe interactions
Drosophila yakuba
2016-12-20T14:58:58Z
2016-12-20T14:58:58Z
en
https://doi.org/10.1534/genetics.116.196238
197520 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Three hybridizing species-the clade ((Drosophila yakuba, D. santomea), D.
teissieri) -comprise the yakuba complex in the D. melanogaster subgroup.
Their ranges overlap on Bioko and São Tomé, islands off west Africa. All
three species are infected with Wolbachia, maternally inherited,
endosymbiotic bacteria, best known for manipulating host reproduction to
favor infected females. Previous analyses reported no cytoplasmic
incompatibility (CI) in these species. However, we discovered that
Wolbachia from each species cause intra- and interspecific CI. In D.
teissieri, analyses of F1 and backcross genotypes show that both host
genotype and Wolbachia variation modulate CI intensity. Wolbachia-infected
females seem largely protected from intra- and interspecific CI,
irrespective of Wolbachia and host genotypes. Wolbachia do not affect host
mating behavior or female fecundity, within or between species. The latter
suggests little apparent effect of Wolbachia on premating or gametic RI
between host species. In nature, Wolbachia frequencies varied spatially
for D. yakuba in 2009, with 76% (N = 155) infected on São Tomé, and only
3% (N = 36) infected on Bioko; frequencies also varied temporally in D.
yakuba and D. santomea on São Tomé between 2009 and 2015. These temporal
frequency fluctuations could generate asymmetries in interspecific mating
success, and contribute to postzygotic RI. However, the fluctuations in
Wolbachia frequencies that we observe also suggest that asymmetries are
unlikely to persist. Finally, we address theoretical questions that our
empirical findings raise about Wolbachia persistence when conditions
fluctuate and about the stable coexistence of Wolbachia and host variants
that modulate Wolbachia effects.
CI_scanData related to population scans for cytoplasmic incompatibility
(CI).Fecundity_scanData related to population scans for Wolbachia effects
on host fecundity.teis_CI_natural_backgroundsData related to analysis of
CI in D. teissieri with Wolbachia in their natural host
backgrounds.teissieri_F1_CIData related to analysis of CI in D. teissieri
with Wolbachia in F1 backgrounds.teis_backcrossData related to analysis of
CI in D. teissieri with Wolbachia backcrossed into different host
backgrounds.teis_CI_ixiData related to analysis of female protection from
CI in D. teissieri.eggs_laid_and_viabilityData related to analysis of
Wolbachia effects on intraspecific and interspecific fecundity and egg to
adult viability in all three host species.infection_statusData related to
analysis of Wolbachia infection frequencies in all three host species, in
Bioko and Sao Tome.prop_matedData related to analysis of Wolbachia effects
on intraspecific and interspecific mating in all three host species.
Sao Tome
United States
Africa
Bioko