10.5061/DRYAD.V54V8
Lebreton, Sebastien
Swedish University of Agricultural Sciences
Borrero-Echeverry, Felipe
Colombian Corporation for Agricultural Research
Gonzalez, Francisco
Swedish University of Agricultural Sciences
Solum, Marit
Swedish University of Agricultural Sciences
Wallin, Erika A.
Mid Sweden University
Hedenström, Erik
Mid Sweden University
Hansson, Bill S.
Max Planck Institute for Chemical Ecology
Gustavsson, Anna-Lena
Science for Life Laboratory
Bengtsson, Marie
Swedish University of Agricultural Sciences
Birgersson, Göran
Swedish University of Agricultural Sciences
Walker III, William B.
Swedish University of Agricultural Sciences
Dweck, Hany K. M.
Max Planck Institute for Chemical Ecology
Becher, Paul G.
Swedish University of Agricultural Sciences
Witzgall, Peter
Swedish University of Agricultural Sciences
Data from: A Drosophila female pheromone elicits species-specific
long-range attraction via an olfactory channel with dual specificity for
sex and food
Dryad
dataset
2018
olfaction
Sexual communication
2018-09-12T00:00:00Z
2018-09-12T00:00:00Z
en
https://doi.org/10.1186/s12915-017-0427-x
74149 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: Mate finding and recognition in animals evolves during niche
adaptation and involves social signals and habitat cues. Drosophila
melanogaster and related species are known to be attracted to fermenting
fruit for feeding and egg-laying, which poses the question of whether
species-specific fly odours contribute to long-range premating
communication. Results: We have discovered an olfactory channel in D.
melanogaster with a dual affinity to sex and food odorants. Female flies
release a pheromone, (Z)-4-undecenal (Z4-11Al), that elicits flight
attraction in both sexes. Its biosynthetic precursor is the cuticular
hydrocarbon (Z,Z)-7,11-heptacosadiene (7,11-HD), which is known to afford
reproductive isolation between the sibling species D. melanogaster and D.
simulans during courtship. Twin olfactory receptors, Or69aB and Or69aA,
are tuned to Z4-11Al and food odorants, respectively. They are
co-expressed in the same olfactory sensory neurons, and feed into a neural
circuit mediating species-specific, long-range communication; however, the
close relative D. simulans, which shares food resources with D.
melanogaster, does not respond to Z4-11Al. Conclusion: The Or69aA and
Or69aB isoforms have adopted dual olfactory traits. The underlying gene
yields a collaboration between natural and sexual selection, which has the
potential to drive speciation.
lebreton data files readmeReadme file, contains data file names and short
description of their contentslebreton 2 chemical analysisChemical analysis
(Table 1); Excel.lebreton 3 ssr screeningSingle sensillum screening, all
sensillum types, native antenna (Figure 1D); Excel.lebreton 4 ssr ab9
ab3Single sensillum recordings in ab9 (native) and ab3 sensilla (following
heterologous expression) (Figure 2A,B); Excel.lebreton 5 strain
energiesPairwise compound comparison (Figure 2C); Excel.lebreton 6 wind
tunnelWind tunnel bioassay (Figure 3); Excel.lebreton 7 OR69a sequenced
transgenesSequences of OR69a variants used to produce transgenic “empty
neuron” flies (Figure 2B). These are 99-100% identical to sequences in the
NCBI database. Text file.lebreton 8 oxidationOxidation of 7,11-HD on glass
(Table 2); Excel.lebreton 1 spectrum tabulatedMass spectrum Z4-11Al,
tabulated (Figure 1A); Excel.