10.5061/DRYAD.V2Q3V
Wale, Nina
Pennsylvania State University
Sim, Derek G.
Pennsylvania State University
Jones, Matthew J.
Pennsylvania State University
Salathe, Rahel
Pennsylvania State University
Day, Troy
Queen's University
Read, Andrew F.
Pennsylvania State University
Data from: Resource limitation prevents the emergence of drug resistance
by intensifying within-host competition
Dryad
dataset
2018
Mus musculus
combination therapy
Drug resistance
Plasmodium chabaudi
2018-12-04T00:00:00Z
2018-12-04T00:00:00Z
en
https://doi.org/10.1073/pnas.1715874115
405272 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Slowing the evolution of antimicrobial resistance is essential if we are
to continue to successfully treat infectious diseases. Whether a
drug-resistant mutant grows to high densities, and so sickens the patient
and spreads to new hosts, is determined by the competitive interactions it
has with drug-susceptible pathogens within the host. Competitive
interactions thus represent a good target for resistance management
strategies. Using an in vivo model of malaria infection, we show that
limiting a resource that is disproportionately required by resistant
parasites retards the evolution of drug resistance by intensifying
competitive interactions between susceptible and resistant parasites.
Resource limitation prevented resistance emergence regardless of whether
resistant mutants arose de novo or were experimentally added before drug
treatment. Our work provides proof of principle that chemotherapy paired
with an “ecological” intervention can slow the evolution of resistance to
antimicrobial drugs, even when resistant pathogens are present at high
frequencies. It also suggests that a broad range of previously untapped
compounds could be used for treating infectious diseases.
Data from Experiment 1 of Wale et al. 2017, PNAS.Excel file containing
(sheet 1) raw data from Experiment 1 & (sheet 2) information
describing the contents.Experiment1.xlsxData from Experiment 2 of Wale et
al. 2017, PNAS.Excel file containing (sheet 1) raw data from Experiment 2
& (sheet 2) information describing the
contents.Experiment2.xlsxData from Experiment 3 of Wale et al. 2017,
PNAS.Excel file containing (sheet 1) raw data from Experiment 3 &
(sheet 2) information describing the contents.Experiment3.xlsxData from
Experiment 4 of Wale et al. 2017, PNAS.Excel file containing (sheet 1) raw
data from Experiment 4 & (sheet 2) information describing the
contents.Experiment4.xlsx