10.5061/DRYAD.TT16KR6
Ravn, Mathias V.
Aarhus University
Campbell, Jacob B.
Arizona State University
Gerber, Lucie
Aarhus University
Harrison, Jon F.
Arizona State University
Overgaard, Johannes
Aarhus University
Data from: Effects of anoxia on ATP, water, ion and pH balance in an
insect (Locusta migratoria)
Dryad
dataset
2019
ion homeostasis
Anoxic stress
Anoxia tolerance
Extracellular potassium
Locusta migratoria
2019-01-15T18:22:13Z
2019-01-15T18:22:13Z
en
https://doi.org/10.1242/jeb.190850
35878 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
When exposed to anoxia insects rapidly go into a hypometabolic coma from
which they can recover when exposed to normoxia again. However, prolonged
anoxic bouts eventually lead to death in most insects, although some
species are surprisingly tolerant. Anoxia challenges ATP, ion, pH and
water homeostasis, but it is not clear how fast and to what degree each of
these parameters are disrupted during anoxia, nor how quickly they
recover. Further, it has not been investigated which disruptions are the
primary source of the tissue damage that ultimately causes death. Here we
show, in the migratory locust (Locusta migratoria), that prolonged anoxic
exposures are associated with increased recovery time, decreased survival,
rapidly disrupted ATP and pH homeostasis and a more slowly disruption of
ion ([K+] and [Na+] ) and water balance. Locusts could not fully recover
after 4 hours of anoxia at 30 °C, and at this point hemolymph [K+] and
[Na+] was elevated 5-fold and decreased 2-fold, respectively, muscle [ATP]
was decreased to ≤3% of normoxic values, hemolymph pH had dropped 0.8
units from 7.3 to 6.5, and hemolymph water content was halved. These
physiological changes are associated with marked tissue damage in vivo and
we show that the isolated and combined effects of hyperkalemia, acidosis
and anoxia can all cause muscle tissue damage in vitro to equally large
degrees. When locusts were returned to normoxia after a moderate (2 hour)
exposure of anoxia, ATP recovered rapidly (15 min) and this was quickly
followed by recovery of ion balance (30 min), while pH recovery took 2-24
hours. Recovery of [K+] and [Na+] coincided with the animals exiting the
comatose state, but recovery to an upright position took∼90 min and was
not related to any of the physiological parameters examined.
Raw data for figuresData_DRYAD.xlsx