10.5061/DRYAD.TQJQ2BVTV
Noh, Suegene
0000-0002-7242-2718
Colby College
Christopher, Lauren
N/A
Strassmann, Joan
Washington University in St. Louis
Queller, David
Washington University in St. Louis
Wild Dictyostelium discoideum social amoebae show plastic responses to the
presence of nonrelatives during multicellular development
Dryad
dataset
2019
FOS: Biological sciences
Dictyostelium discoideum
social conflict
group size
Division of Integrative Organismal Systems
https://ror.org/01rvays47
12,564,161,656,756
2021-01-18T00:00:00Z
2021-01-18T00:00:00Z
en
https://doi.org/10.1002/ece3.5924
https://doi.org/10.1073/pnas.1720324115
17261443 bytes
3
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
When multiple strains of microbes form social groups, such as the
multicellular fruiting bodies of Dictyostelium discoideum, conflict can
arise regarding cell fate. Both fixed and plastic differences among
strains can contribute to cell fate, and plastic responses may be
particularly important if social environments frequently change. We used
RNA-sequencing and photographic time series analysis to detect possible
conflict-induced plastic differences between wild D. discoideum aggregates
formed by single strains compared to mixed pairs of strains (chimeras). We
found one hundred and two differentially expressed genes that were
enriched for biological processes including cytoskeleton organization and
cyclic-AMP response (up-regulated in chimeras), and DNA replication and
cell cycle (down-regulated in chimeras). In addition, our data indicate
that in reference to a time series of multicellular development in the lab
strain AX4, chimeras may be slightly behind clonal aggregates in their
development. Finally, phenotypic analysis supported slower splitting of
aggregates and a nonsignificant trend for larger group sizes in chimeras.
The transcriptomic comparison and phenotypic analyses support
discoordination among aggregate group members due to social conflict.
These results are consistent with previously observed factors that affect
cell fate decision in D. discoideum and provide evidence for plasticity in
cAMP signaling and phenotypic coordination during development in response
to social conflict in D. discoideum and similar microbial social groups.