10.5061/DRYAD.TF536
Preite, Veronica
Nederlands Instituut voor Ecologie
Oplaat, Carla
Nederlands Instituut voor Ecologie
Biere, Arjen
Nederlands Instituut voor Ecologie
Kirschner, Jan
Academy of Sciences of the Czech Republic
van der Putten, Wim H.
Wageningen University & Research
Nederlands Instituut voor Ecologie
Verhoeven, Koen J. F.
Nederlands Instituut voor Ecologie
Data from: Increased transgenerational epigenetic variation, but not
predictable epigenetic variants, after environmental exposure in two
apomictic dandelion lineages
Dryad
dataset
2019
stress memory
DNA methylation
Taraxacum officinale
Taraxacum officinale hemicyclum
Salicylic acid
Taraxacum officinale alatum
2019-01-12T00:00:00Z
2019-01-12T00:00:00Z
en
https://doi.org/10.1002/ece3.3871
161257 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
DNA methylation is one of the mechanisms underlying epigenetic
modifications. DNA methylations can be environmentally induced and such
induced modifications can at times be transmitted to successive
generations. However, it remains speculative how common such
environmentally induced transgenerational DNA methylation changes are and
if they persist for more than one offspring generation. We exposed
multiple accessions of two different apomictic dandelion lineages of the
Taraxacum officinale group (Taraxacum alatum and T. hemicyclum) to drought
and salicylic acid (SA) treatment. Using methylation-sensitive amplified
fragment length polymorphism markers (MS-AFLPs) we screened anonymous
methylation changes at CCGG restriction sites throughout the genome after
stress treatments and assessed the heritability of induced changes for two
subsequent unexposed offspring generations. Irrespective of the initial
stress treatment, a clear buildup of heritable DNA methylation variation
was observed across three generations, indicating a considerable
background rate of heritable epimutations. Less evidence was detected for
environmental effects. Drought stress showed some evidence for
accession-specific methylation changes, but only in the exposed generation
and not in their offspring. By contrast, SA treatment caused an increased
rate of methylation change in offspring of treated plants. These changes
were seemingly undirected resulting in increased transgenerational
epigenetic variation between offspring individuals, but not in predictable
epigenetic variants. While the functional consequences of these
MS-AFLP-detected DNA methylation changes remain to be demonstrated, our
study shows that (1) stress-induced transgenerational DNA methylation
modification in dandelions is genotype and context-specific; and (2)
inherited environmental DNA methylation effects are mostly undirected and
not targeted to specific loci.
Preite_etal_2018_EcolEvol_HpaII_Rawdata_and_ConsensusData from: Preite et
al. 2018 (DOI: 10.1002/ece3.3871) Increased transgenerational epigenetic
variation, but not predictable epigenetic variants, after environmental
exposure in two apomictic dandelion lineages. Excel file shows HpaII raw
data (methylation sensitive -AFLPs), consensus and deviation from
consensus across generations.
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