10.5061/DRYAD.T671S
Roschanski, Anna M.
Conservation Biology; Faculty of Biology; University of Marburg;
Karl-von-Frisch-Straße Marburg 35032 Germany
Csilléry, Katalin
Ecologie des Forêts Méditerranéennes
Liepelt, Sascha
Conservation Biology; Faculty of Biology; University of Marburg;
Karl-von-Frisch-Straße Marburg 35032 Germany
Oddou-Muratorio, Sylvie
Ecologie des Forêts Méditerranéennes
Ziegenhagen, Birgit
Ecologie des Forêts Méditerranéennes
Huard, Frédéric
French National Institute for Agricultural Research
Ullrich, Kristian K.
Sant'Anna School of Advanced Studies
Postolache, Dragos
Institute of Biosciences and Bioresources
Forest Research
Vendramin, Giovanni G.
Institute of Biosciences and Bioresources
Fady, Bruno
Ecologie des Forêts Méditerranéennes
Data from: Evidence of divergent selection for drought and cold tolerance
at landscape and local scales in Abies alba Mill. in the French
Mediterranean Alps
Dryad
dataset
2015
landscape genomics
Abies alba
candidate gene
European silver fir
Holocene
2015-12-14T15:13:17Z
2015-12-14T15:13:17Z
en
https://doi.org/10.1111/mec.13516
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Understanding local adaptation in forest trees is currently a key research
and societal priority. Geographically and ecologically marginal
populations provide ideal case studies, because environmental stress along
with reduced gene flow can facilitate the establishment of locally adapted
populations. We sampled European silver fir (Abies alba Mill.) trees in
the French Mediterranean Alps, along the margin of its distribution range,
from pairs of high and low elevation plots on four different mountains
situated along a 170 km east-west transect. The analysis of 267 SNP loci
from 175 candidate genes suggested a neutral pattern of east-west
isolation-by-distance among mountain sites. FST outlier tests revealed 16
SNPs that showed patterns of divergent selection. Plot climate was
characterized using both in-situ measurements and gridded data that
revealed marked differences between and within mountains with different
trends depending on the season. Association between allelic frequencies
and bio-climatic variables revealed eight genes that contained candidate
SNPs of which two were also detected using FST outlier methods. All SNPs
were associated with winter drought and one of them showed strong evidence
of selection with respect to elevation. QST - FST tests for fitness
related traits measured in a common garden, suggested adaptive divergence
for the date of bud flush and for growth rate. Overall, our results
suggest a complex adaptive picture for A. alba in the southern French Alps
where, during the east to west Holocene recolonization, locally
advantageous genetic variants established both at the landscape and local
scales.
common_garden_data_and_Qst-Fst_testsA series of files containing common
garden data (budburst, survival and growth) from a nursery tests (see
Sagnard et al. 2002) and R scripts to perform the Qst-Fst tests with the
QstFstComp packageenvironmental_dataA list of environmental variables
characterizing the four studied sites from southern France (Ventoux, Lure,
Issole and Vésubie)IBE_vs_IBD_testsR scripts to perform the Mantel tests
for detecting isolation-by-distance and isolation by-environment patterns
using the ecodist packageKASP_detailsSequences (raw data) on which primer
design for KASP assay was based and SNPs thereinoutlier_testsResults of
outlier tests using Bayescan, FLK, hFDIST and LFMM, and R scripts for
running LFMM using the LEA packagesampling_sitesLambert 3 coordinates and
altitude of all sampled individualsSTRUCTUREShell scripts and results of
the STRUCTURE analysis with K=1 to 9SNP_dataRaw SNP data called from
transcriptome accession no.: JV134525–JV157085 TSA NCBI, and inferred SNP
data using the software PHASE
Mediterranean
Issole
Vésubie
Ventoux
Lure
southern Europe
France