10.5061/DRYAD.SB68V
Vanden Broeck, An
Research Institute for Nature and Forest
Van Landuyt, Wouter
Research Institute for Nature and Forest
Cox, Karen
Research Institute for Nature and Forest
De Bruyn, Luc
Research Institute for Nature and Forest
Gyselings, Ralf
Research Institute for Nature and Forest
Oostermeijer, Gerard
University of Amsterdam
Valentin, Bertille
Conservatoire Botanique National de Bailleul, Hameau de Haendries,
Bailleul, France
Božič, Gregor
Slovenian Forestry Institute
Dolinar, Branko
Botanical Society of Slovenia, Ljubljana, Slovenia
Illyés, Zoltán
Zalaegerszeg, Hungary
Mergeay, Joachim
Research Institute for Nature and Forest
Data from: High levels of effective long-distance dispersal may blur
ecotypic divergence in a rare terrestrial orchid
Dryad
dataset
2014
long-distance seed dispersal
Orchidaceae
Liparis loeselii L.
amplified fragment length polymorphism (AFLP)
ecotypic divergence
Holocene
amplified fragment length polymorphism (AFLP)
2014-07-08T17:42:50Z
2014-07-08T17:42:50Z
en
https://doi.org/10.1186/1472-6785-14-20
416564 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: Gene flow and adaptive divergence are key aspects of
metapopulation dynamics and ecological speciation. Long-distance dispersal
is hard to detect and few studies estimate dispersal in combination with
adaptive divergence. The aim of this study was to investigate effective
long-distance dispersal and adaptive divergence in the fen orchid (Liparis
loeselii (L.) Rich.). We used amplified fragment length polymorphism
(AFLP)-based assignment tests to quantify effective long-distance
dispersal at two different regions in Northwest Europe. In addition,
genomic divergence between fen orchid populations occupying two
distinguishable habitats, wet dune slacks and alkaline fens, was
investigated by a genome scan approach at different spatial scales
(continental, landscape and regional) and based on 451 AFLP loci. Results:
We expected that different habitats would contribute to strong divergence
and restricted gene flow resulting in isolation-by-adaptation. Instead, we
found remarkably high levels of effective long-distance seed dispersal and
low levels of adaptive divergence. At least 15% of the assigned
individuals likely originated from among-population dispersal events with
dispersal distances up to 220 km. Six (1.3%) ‘outlier’ loci, potentially
reflecting local adaptation to habitat-type, were identified with high
statistical support. Of these, only one (0.22%) was a replicated outlier
in multiple independent dune-fen population comparisons and thus possibly
reflecting truly parallel divergence. Signals of adaptation in response to
habitat type were most evident at the scale of individual populations.
Conclusions: The findings of this study suggest that the homogenizing
effect of effective long-distance seed dispersal may overwhelm divergent
selection associated to habitat type in fen orchids in Northwest Europe.
AFLP dataThe file AFLP data.txt includes the binary matrix of AFLP band
presence (1) – absence (0) for 451 AFLP-markers and 422 individuals of
Liparis loeselii sampled in 38 populations. The matrix also include the
spatial coordinates of each sampled population, given in the two last
columns. The markers are numbered M1 to M451. The description of each
marker (size in base pairs, primer combination) is given in the file
readme.txt.
Europe