10.5061/DRYAD.S95F1
Li, Weihan
Howard Hughes Medical Institute
University of California System
Okreglak, Voytek
Howard Hughes Medical Institute
University of California System
Peschek, Jirka
Howard Hughes Medical Institute
University of California System
Kimmig, Philipp
Howard Hughes Medical Institute
University of California System
Zubradt, Meghan
Howard Hughes Medical Institute
University of California System
Weissman, Jonathan S.
University of California System
Walter, Peter
Howard Hughes Medical Institute
University of California System
Data from: Engineering ER-stress dependent non-conventional mRNA splicing
Dryad
dataset
2019
DMS-seq
Schizosaccharomyces pombe
RNA structure
National Science Foundation
https://ror.org/021nxhr62
1144247
2019-06-19T00:00:00Z
2019-06-19T00:00:00Z
en
https://doi.org/10.7554/elife.35388
2732797368 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The endoplasmic reticulum (ER) protein folding capacity is balanced with
the protein folding burden to prevent accumulation of un- or misfolded
proteins. The ER membrane-resident kinase/RNase Ire1 maintains ER protein
homeostasis through two fundamentally distinct processes. First, Ire1 can
initiate a transcriptional response through a non-conventional mRNA
splicing reaction to increase the ER folding capacity. Second, Ire1 can
decrease the ER folding burden through selective mRNA decay. In
Saccharomyces cerevisiae and Schizosaccharomyces pombe, the two Ire1
functions have been evolutionarily separated. Here, we show that the
respective Ire1 orthologs have become specialized for their functional
outputs by divergence of their RNase specificities. In addition, RNA
structural features separate the splicing substrates from the decay
substrates. Using these insights, we engineered an S. pombe Ire1 cleavage
substrate into a splicing substrate, which confers S. pombe with both Ire1
functional outputs.
MegZ03_index13_AGTCAA_1MISS_lowerM_wildtype_polyA_PLUSstrand_COUNTSThis is
.WIG file (processed from .fastq files) for DMS-seq data in S. pombe cells
(plus strand) (generated by Meghan
Zubradt)MegZ03_index13_AGTCAA_1MISS_lowerM_wildtype_polyA_MINUSstrand_COUNTSThis is .WIG file (processed from .fastq files) for DMS-seq data in S. pombe cells (minus strand) (generated by Meghan Zubradt)MegZ03_index13_AGTCAA_L005_R1_001.fastq.gzThis is the unprocessed sequencing .fastq file (compressed) for S. pombe DMS-seq experiment (generated by Meghan Zubradt)