10.5061/DRYAD.RK7N8
Graça, Rodrigo N.
Washington State University
Ross-Davis, Amy L.
Rocky Mountain Research Station
Klopfenstein, Ned B.
Rocky Mountain Research Station
Kim, Mee-Sook
Kookmin University
Peever, Tobin L.
Washington State University
Cannon, Philip G.
United States Department of Agriculture
Aun, Cristina P.
Universidade Federal de Viçosa
Mizubuti, Eduardo S. G.
Universidade Federal de Viçosa
Alfenas, Acelino C.
Universidade Federal de Viçosa
Data from: Rust disease of eucalypts, caused by Puccinia psidii, did not
originate via host jump from guava in Brazil
Dryad
dataset
2013
Puccinia psidii
host jump
Host Parasite Interactions
emerging disease
2013-09-26T17:26:23Z
2013-09-26T17:26:23Z
en
https://doi.org/10.1111/mec.12545
27534 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The rust fungus, Puccinia psidii, is a devastating pathogen of introduced
eucalypts (Eucalyptus spp.) in Brazil where it was first observed in 1912.
This pathogen is hypothesized to be endemic to South and Central America
and to have first infected eucalypts via a host jump from native guava
(Psidium guajava). Ten microsatellite markers were used to genotype 148 P.
psidii samples from eucalypts and guava plus five additional myrtaceous
hosts across a wide geographic range of southeastern Brazil and Uruguay.
Principal coordinates analysis, a Bayesian clustering analysis, and a
minimum spanning network revealed two major genetic clusters among the
sampled isolates, one associated with guava and another associated with
eucalypts and three additional hosts. Multilocus genotypes infecting guava
differed by multiple mutational steps at eight loci compared to those
infecting eucalypts. Approximate Bayesian computation revealed that
evolutionary scenarios involving a coalescence event between guava- and
eucalypt-associated pathogen populations within the past 1000 years to be
highly unlikely. Divergence time estimates conducted over a wide range of
demographic scenarios indicated that the population split between
eucalypt- and guava-infecting pathogens occurred approximately 15,000 to
150,000 years ago. None of the analyses supported the hypothesis that
eucalypt-infecting P. psidii in Brazil originated via host jump from guava
since the introduction of eucalypts to Brazil approximately 185 years ago.
The existence of host-associated biotypes of P. psidii in Brazil indicates
that this diversity must be considered when assessing the invasive threat
posed by this pathogen to myrtaceous hosts worldwide.
Geographic coordinates and allele scores of Puccinia psidii samples from
Brazil and UruguayData include geographic coordinates of single uredinial
pustules of Puccinia psidii ca. 6 mm diameter collected between March 2008
and August 2009 from 148 individual plants representing seven myrtaceous
taxa (eucalypts, guava, rose apple, Brazilian guava, Java plum,
jabuticaba, and pitanga) in nine Brazilian states and one location in
Uruguay. Samples were genotyped at 10 microsatellite loci (PpSSR012,
PpSSR014, PpSSR018, PpSSR022, PpSSR087, PpSSR102, PpSSR146, PpSSR161,
PpSSR178, and PpSSR195) using genomic DNA extracted directly from each
pustule following a modified CTAB-based protocol. Fragment analysis was
via capillary electrophoresis using an ABI 3700 DNA automated sequencer.
Positive and negative controls were included for each locus scored and
scoring was repeated for representative alleles for each locus to ensure
the accuracy of genotyping. Allele sizes were estimated using marker
standards (ROX Geneflo 625, CHIMERx, Milwaukee, WI, USA) and scored using
ABI PeakScanner Analysis Software v1.0.DRYAD.xlsx
Uruguay
Brazil