10.5061/DRYAD.R9693
Altamia, Marvin A.
Oregon Health & Science University
Wood, Nicole
Northeastern University
Fung, Jennifer M.
Laboratory for Marine Genomic Research; Ocean Genome Legacy Inc.;
Ipswich MA 01938 USA
Dedrick, Sandra
Central Michigan University
Linton, Eric W.
Central Michigan University
Concepcion, Gisela P.
University of the Philippines Diliman
Haygood, Margo G.
Oregon Health & Science University
Distel, Daniel L.
Northeastern University
Data from: Genetic differentiation among isolates of Teredinibacter
turnerae, a widely occurring intracellular endosymbiont of shipworms
Dryad
dataset
2014
Symbiosis
Bivalvia:Teredinidae
recombination
Bivalvia
Shipworm
Teredinidae
Teredinibacter
lateral gene transfer
2014-01-14T15:24:44Z
2014-01-14T15:24:44Z
en
https://doi.org/10.1111/mec.12667
119542 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Teredinibacter turnerae is a cultivable intracellular endosymbiont of
xylotrophic (wood-feeding) bivalves of the Family Teredinidae (shipworms).
Although T. turnerae has been isolated from many shipworm taxa collected
in many locations, no systematic effort has been made to explore genetic
diversity within this symbiont species across the taxonomic and
geographical range of its hosts. The mode of symbiont transmission is
unknown. Here, we examine sequence diversity in fragments of six genes
(16S rRNA, gyrB, sseA, recA, rpoB and celAB) among 25 isolates of T.
turnerae cultured from 13 shipworm species collected in 15 locations in
the Atlantic, Pacific and Indian Oceans. While 16S rRNA sequences are
nearly invariant between all examined isolates (maximum pairwise
difference <0.26%), variation between examined protein-coding loci
is greater (mean pairwise difference 2.2–5.9%). Phylogenetic analyses
based on each protein-coding locus differentiate the 25 isolates into two
distinct and well-supported clades. With five exceptions, clade
assignments for each isolate were supported by analysis of alleles of each
of the five protein-coding loci. These exceptions include (i) putative
recombinant alleles of the celAB and gyrB loci in two isolates
(PMS-535T.S.1b.3 and T8510), suggesting homologous recombination between
members of the two clades; and (ii) evidence for a putative lateral gene
transfer event affecting a second locus (recA) in three isolates (T8412,
T8503 and T8513). These results demonstrate that T. turnerae isolates do
not represent a homogeneous global population. Instead, they indicate the
emergence of two lineages that, although distinct, likely experience some
level of genetic exchange with each other and with other bacterial
species.
Figure 1 Bayesian treesZIP file containing Figure 1 Bayesian trees and
alignments in Nexus format.Figure S2 Bayesian treesZIP file containing
Figure S2 trees and alignments in Nexus format.Figure S3 ML treesZIP file
containing Figure S3 Maximum Likelihood trees and alignments in Nexus
format.Figure S4_recA1 A2 Bayesian treeFigure S4 tree and alignment in
Nexus format.recA of Teredinibacter turnerae strains T8412, T8503, and
T8513Sequence alignment of recA loci of Teredinibacter turnerae strains
T8412, T8503, and T8513