10.5061/DRYAD.QS4NH
Mazer, Susan J.
University of California, Santa Barbara
Hendrickson, Brandon Thomas
University of California, Santa Barbara
Chellew, Joseph P.
University of California, Santa Barbara
Kim, Lynn J.
University of California, Santa Barbara
Liu, Jasen W.
University of California, Santa Barbara
Shu, Jasper
University of California, Santa Barbara
Sharma, Manju V.
University of California, Santa Barbara
Mazer, Susan
University of California, Santa Barbara
Data from: Divergence in pollen performance between Clarkia sister species
with contrasting mating systems supports predictions of sexual selection
Dryad
dataset
2018
pollen tube growth rate
Mating System evolution
gametophytic selection
Onagraceae
pollen competition
Clarkia unguiculata
Clarkia exilis
sexual selction
Holocene
2018-01-24T14:55:21Z
2018-01-24T14:55:21Z
en
https://doi.org/10.1111/evo.13429
986624 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Animal taxa that differ in the intensity of sperm competition often differ
in sperm production or swimming speed, arguably due to sexual selection on
postcopulatory male traits affecting siring success. In plants, closely
related self- and cross-pollinated taxa similarly differ in the
opportunity for sexual selection among male gametophytes after
pollination, so traits such as the proportion of pollen on the stigma that
rapidly enters the style and mean pollen tube growth rate (PTGR) are
predicted to diverge between them. To date, no studies have tested this
prediction in multiple plant populations under uniform conditions. We
tested for differences in pollen performance in greenhouse-raised
populations of two Clarkia sister species: the predominantly outcrossing
C. unguiculata and the facultatively self-pollinating C. exilis. Within
populations of each taxon, groups of individuals were reciprocally
pollinated (n = 1153 pollinations) and their styles examined four hours
later. We tested for the effects of species, population, pollen type (self
vs. outcross), the number of competing pollen grains, and temperature on
pollen performance. Clarkia unguiculata exhibited higher mean PTGR than C.
exilis; pollen type had no effect on performance in either taxon. The
difference between these species in PTGR is consistent with predictions of
sexual selection theory.
Observations on individual styles (n=1153 records)Each record in this
spreadsheet includes the observations recorded for an individual style,
including whether it was self- vs. cross-pollinated, the number of pollen
grains adhering to the stigma, and the number of pollen tubes visible in
each 1-mm interval of the style four hours following pollination. The
temperature recorded at the time of pollination and the mean temperature
recorded for the four hours following pollination are also included. The
stigma penetrance rate and the mean pollen tube growth rate among all
pollen tubes visible in the style are also estimated based on Equation 1
in the published manuscript.IndividualStyles_N_of_1153.xlsPollen Donor
Means by Pollination Type (Selfed vs Outcrossed Pollinations) (N=254)Each
of the 254 records in this spreadsheet includes the mean values for the
observations recorded for the selfed or the outcrossed flowers of a given
pollen donor. In a very few cases, a pollen donor is represented by only
one type of pollination. This data set was used in all of the analyses
reported in the published article and in the Supplemental Materials for
which the sample size was ~254 (in some analyses, the sample size was
reduced due to missing
values).PollenDonorMeansByPollinationType_SelfedVsOutcrossed_N_of_254.xlsPollen Donor Means (Means of Selfed and Outcrossed Pollinations) (N=136)Each of the 136 records in this spreadsheet includes the mean values for the observations recorded for the selfed AND the outcrossed flowers of a given pollen donor. In other words, these values are the means of the two means (i.e., the mean of the selfed flowers and the mean of the outcrossed flowers). This data set was used in all of the analyses in the Supplemental Materials for which the sample size was ~136 (in some analyses, the sample size was reduced due to missing values).PollenDonorMeans_MeanOfSelfedAndOutcrossed_N_of_136.xls
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