10.5061/DRYAD.QH03C
González, Candela R.
Universidad Maimónides
González, Betina
University of Buenos Aires
Matzkin, Maria E.
Experimental Medicine and Biology Institute
Muñiz, Javier A.
University of Buenos Aires
Cadet, Jean Lud
National Institute on Drug Abuse
Garcia-Rill, Edgar
University of Arkansas for Medical Sciences
Urbano, Francisco J.
University of Buenos Aires
Vitullo, Alfredo D.
Universidad Maimónides
Bisagno, Veronica
University of Buenos Aires
Data from: Psychostimulant-induced testicular toxicity in mice: evidence
of cocaine and caffeine effects on the local dopaminergic system
Dryad
dataset
2016
Adenosine
Caffeine
Cocaine
Spermatogenesis
Dopamine
tyrosine hydroxylase
2016-02-22T16:20:46Z
2016-02-22T16:20:46Z
en
https://doi.org/10.1371/journal.pone.0142713
35187 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Several organ systems can be affected by psychostimulant toxicity.
However, there is not sufficient evidence about the impact of
psychostimulant intake on testicular physiology and catecholaminergic
systems. The aim of the present study was to further explore potential
toxic consequences of chronic exposure to cocaine, caffeine, and their
combination on testicular physiology. Mice were injected with a 13-day
chronic binge regimen of caffeine (3x5mg/kg), cocaine (3×10mg/kg), or
combined administration. Mice treated with cocaine alone or combined with
caffeine showed reduced volume of the seminiferous tubule associated to a
reduction in the number of spermatogonia. Cocaine-only and combined
treatments induced increased lipid peroxidation evaluated by TBARS assay
and decreased glutathione peroxidase mRNA expression. Importantly,
caffeine-cocaine combination potentiated the cocaine-induced germ cell
loss, and induced pro-apoptotic BAX protein expression and diminished
adenosine receptor A1 mRNA levels. We analyzed markers of dopaminergic
function in the testis and detected the presence of tyrosine hydroxylase
(TH) in the cytoplasm of androgen-producing Leydig cells, but also in
meiotic germs cells within seminiferous tubules. Moreover, using
transgenic BAC-Drd1a-tdTomato and D2R-eGFP mice, we report for the first
time the presence of dopamine receptors (DRs) D1 and D2 in testicular
mouse Leydig cells. Interestingly, the presence of DRD1 was also detected
in the spermatogonia nearest the basal lamina of the seminiferous tubules,
which did not show TH staining. We observed that psychostimulants induced
downregulation of DRs mRNA expression and upregulation of TH protein
expression in the testis. These findings suggest a potential role of the
local dopaminergic system in psychostimulant-induced testicular pathology.
Gonzalez et al Dataraw data and statistical analysisGonzalez et al Dryad.xlsx