10.5061/DRYAD.QF6T5
Percy, Diana M.
University of British Columbia
Argus, George W.
Canadian Museum of Nature
Cronk, Quentin C.
University of British Columbia
Fazekas, Aron J.
University of Guelph
Kesanakurti, Prasad R.
University of Guelph
Burgess, Kevin S.
Columbus State University
Husband, Brian C.
University of Guelph
Newmaster, Steven G.
University of Guelph
Barrett, Spencer C. H.
University of British Columbia
Graham, Sean W.
University of British Columbia
Barrett, Spencer C.H.
University of Toronto
Data from: Understanding the spectacular failure of DNA barcoding in
willows (Salix): Does this result from a trans-specific selective sweep?
Dryad
dataset
2014
Salix
Chloroplast capture
specimen records
Malpighiales
2014-06-17T15:35:20Z
2014-06-17T15:35:20Z
en
https://doi.org/10.1111/mec.12837
2269949 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Willows (Salix: Salicaceae) form a major ecological component of Holarctic
floras, and consequently are an obvious target for a DNA-based
identification system. We surveyed two to seven plastid genome regions
(~3.8 kb; ~3% of the genome) from 71 Salix species across all five
subgenera, to assess their performance as DNA barcode markers. Although
Salix has a relatively high level of interspecific hybridization, this may
not sufficiently explain the near complete failure of barcoding that we
observed: only one species had a unique barcode. We recovered 39 unique
haplotypes, from more than 500 specimens, that could be partitioned into
six major haplotype groups. A unique variant of group I (haplotype 1*) was
shared by 53 species in three of five Salix subgenera. This unusual
pattern of haplotype sharing across infrageneric taxa is suggestive of
either a massive non-random coalescence failure (incomplete lineage
sorting), or of repeated plastid capture events, possibly including a
historical selective sweep of haplotype 1* across taxonomic sections. The
former is unlikely as molecular dating indicates that haplotype 1*
originated recently, and is nested in the oldest major haplotype group in
the genus. Further, we detected significant non-neutrality in the
frequency spectrum of mutations in group I, but not outside group I, and
demonstrated a striking absence of geographic structure to the haplotype
distributions in this group. The most likely explanation for the patterns
we observed involves recent repeated plastid capture events, aided by
widespread hybridization and long-range seed dispersal, but primarily
propelled by one or more trans-species selective sweeps.
bold salix samplesspecies, specimen, location listsSALIX_matKmatK sequence
alignmentSALIX_rbcLrbcL sequence alignmentSALIX_rpoBrpoB sequence
alignmentSALIX_rpoC1rpoC1 sequence alignmentSALIX_trnHtrnH-psbA sequence
alignmentSALIX_psbKpsbK-psbI sequence alignmentSALIX_atpFatpF-atpH
sequence alignmentSALIX_COICOI sequence alignment