10.5061/DRYAD.Q57N9H7
Duxbury, Ashley E.
University of Georgia
Weathersby, Brandie
University of Georgia
Sanchez, Zachary
University of Georgia
Moore, Patricia J.
University of Georgia
Data from: A study of the transit amplification divisions during
spermatogenesis in Oncopeltus fasciatus to assess plasticity in sperm
numbers or sperm viability under different diets
Dryad
dataset
2019
fertility
Oncopeltus fasciatus
Spermatogenesis
sperm counts
2019-05-07T00:00:00Z
2019-05-07T00:00:00Z
en
https://doi.org/10.1002/ece3.4511
57943 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Oncopeltus fasciatus males fed the ancestral diet of milkweed seeds
prioritize reproduction over lifespan as evidenced by higher rates of
fertility and shorter lifespans than males from the same population fed
the adapted diet of sunflower seeds. We examined the proximate mechanisms
by which milkweed-fed males maintained late-life fertility. We tested the
hypothesis that older milkweed-fed males maintained fertility by producing
more, higher quality sperm. Our results, that older males have more sperm,
but their sperm do not have higher viability, is in general agreement with
other recent studies on how nutrition affects male fertility in insects.
We further examined the mechanisms by which sperm are produced by
examining the progression of spermatogonial cells through the cell cycle
during the transit amplification divisions. We demonstrated that diet
affects the likelihood of a spermatocyst being in the S-phase or M-phase
of the cell cycle. Given work in model systems, these results have
implications for subtle effects on sperm quality either through
replication stress or epigenetic markers. Thus, viability may not be the
best marker for sperm quality and more work is called for on the
mechanisms by which the germline and the production of sperm mediate the
cost of reproduction.
Fecundity and Fertility data for Figure 4Excel spreadsheet containing data
collected on laboratory populations of Oncopeltus fasciatus. Data was
collected for females mated to males at 2-weeks or 4-weeks post adult
emergence that had been reared on either a diet of sunflower or milkweed
seeds following adult emergence. The total number of eggs laid was
counted. The total number of eggs that showed evidence of fertilization
was also counted and the percent of eggs fertilized was
calculated.fecundity and fertility data.xlsSperm Count data for figure
5Excel spreadsheet with data on sperm counts of males fed a diet of either
milkweed or sunflower seeds from adult emergence. Sperm counts were
calculated from a subsample. Sperm counts estimate the total number of
sperm stored in the seminal vesicle at the time of dissection.Sperm
Counts.xlsxspermatocyst cell division ratesExcel spreadsheet with data on
the number of spermatocysts staining positive for EdU incorporation or
phospho-histone H3 modification. Data taken from the testes of males fed a
diet of milkweed or sunflower seeds after adult emergence.GSC division
rates.xlsx