10.5061/DRYAD.Q4Q52
Alexander, Brittany E.
University of Amsterdam
Liebrand, Kevin
University of Amsterdam
Osinga, Ronald
Porifarma B.V. Poelbos 3, Ede, The Netherlands
van der Geest, Harm G.
University of Amsterdam
Admiraal, Wim
University of Amsterdam
Cleutjens, Jack P. M.
Maastricht University
Schutte, Bert
Maastricht University
Verheyen, Fons
Electron Microscopy Unit, CRISP, Maastricht, The Netherlands
Ribes, Marta
Institut de Ciències del Mar
van Loon, Emiel
University of Amsterdam
de Goeij, Jasper M.
University of Amsterdam
Data from: Cell turnover and detritus production in marine sponges from
tropical and temperate benthic ecosystems
Dryad
dataset
2015
Sponges
Cell proliferation
Scopalina ruetzleri
Clathria sp.
Monanchora arbuscula
Haliclona vansoesti
tissue homeostasis
Myclae microsigmatosa
cell shedding
Chondrilla caribensis
Halisarca caerulea
Chondrosia reniformis
cell turnover
detritus
2015-09-18T00:00:00Z
2015-09-18T00:00:00Z
en
https://doi.org/10.1371/journal.pone.0109486
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
This study describes in vivo cell turnover (the balance between cell
proliferation and cell loss) in eight marine sponge species from tropical
coral reef, mangrove and temperate Mediterranean reef ecosystems. Cell
proliferation was determined through the incorporation of
5-bromo-2′-deoxyuridine (BrdU) and measuring the percentage of
BrdU-positive cells after 6 h of continuous labeling (10 h for Chondrosia
reniformis). Apoptosis was identified using an antibody against active
caspase-3. Cell loss through shedding was studied quantitatively by
collecting and weighing sponge-expelled detritus and qualitatively by
light microscopy of sponge tissue and detritus. All species investigated
displayed substantial cell proliferation, predominantly in the choanoderm,
but also in the mesohyl. The majority of coral reef species (five) showed
between 16.1±15.9% and 19.0±2.0% choanocyte proliferation (mean±SD) after
6 h and the Mediterranean species, C. reniformis, showed 16.6±3.2% after
10 h BrdU-labeling. Monanchora arbuscula showed lower choanocyte
proliferation (8.1±3.7%), whereas the mangrove species Mycale
microsigmatosa showed relatively higher levels of choanocyte proliferation
(70.5±6.6%). Choanocyte proliferation in Haliclona vansoesti was variable
(2.8–73.1%). Apoptosis was negligible and not the primary mechanism of
cell loss involved in cell turnover. All species investigated produced
significant amounts of detritus (2.5–18% detritus bodyweight−1·d−1) and
cell shedding was observed in seven out of eight species. The amount of
shed cells observed in histological sections may be related to differences
in residence time of detritus within canals. Detritus production could not
be directly linked to cell shedding due to the degraded nature of expelled
cellular debris. We have demonstrated that under steady-state conditions,
cell turnover through cell proliferation and cell shedding are common
processes to maintain tissue homeostasis in a variety of sponge species
from different ecosystems. Cell turnover is hypothesized to be the main
underlying mechanism producing sponge-derived detritus, a major trophic
resource transferred through sponges in benthic ecosystems, such as coral
reefs.
Proliferation difference r fileR scripts for percentage of proliferating
choanocyte and mesohyl cells per individual.prolif_diff.rDifference in the
percentage proliferation in choanocytes and mesohyl cellsPercentage of
proliferating choanocyte and mesohyl cells per individual and per
speciesprolif_diff.csvHalisarca caerulea choanocyte proliferation 6h vs
10hPercentage of proliferating choanocytes in Halisarca caerulea
specimens, incubated with BrdU for either 6 hours or 10
hours.prolif_6vs10.csvHalisarca caerulea choanocyte proliferation 6h vs
10h r scriptR script for percentage of proliferating choanocytes in
Halisarca caerulea specimens, incubated with BrdU for either 6 hours or 10
hours.prolif_6vs10.rDetritus productionDaily detritus production (in mg)
for test and control samples, for several sponge species and (for most
individuals) sampling over timedetritus_prod.csvDetritus production R
scriptR script for daily detritus production (in mg) for test and control
samples, for several species and (for most individuals) sampling over
timedetritus_prod.rDetritus production vs shedder typeDaily detritus
production as percentage of body weight for various sponge species and
shedder types.detritus_shed.csvDetritus production vs shedder type R
scriptR script for daily detritus production as percentage of body weight
for various species and shedder types.detritus_shed.r
Curacao
Spain