10.5061/DRYAD.Q2K15
Yasukochi, Yuji
National Institute of Agrobiological Sciences
Ohno, Mizuki
Iwate University
Shibata, Fukashi
Hokkaido University
Jouraku, Akiya
National Institute of Agrobiological Sciences
Nakano, Ryo
University of Tokyo
Ishikawa, Yukio
University of Tokyo
Sahara, Ken
Iwate University
Data from: A FISH-based chromosome map for the European corn borer yields
insights into ancient chromosomal fusions in the silkworm
Dryad
dataset
2015
Ostrinia furnacalis
Scaffold and singleton contig sequences
2015-07-07T16:17:36Z
2015-07-07T16:17:36Z
en
https://doi.org/10.1038/hdy.2015.72
162828055 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
A significant feature of the genomes of Lepidoptera, butterflies and
moths, is the high conservation of chromosome organization. Recent
remarkable progress in genome sequencing of Lepidoptera has revealed that
syntenic gene order is extensively conserved across phylogenetically
distant species. The ancestral karyotype of Lepidoptera is thought to be n
= 31; however, that of the most well studied moth, Bombyx mori, is n = 28,
and diverse studies suggest that three chromosomal fusion events occurred
in this lineage. To identify the boundaries between predicted ancient
fusions involving B. mori chromosomes 11, 23 and 24, we constructed
FISH-based chromosome maps of the European corn borer, Ostrinia nubilalis
(n = 31). We first determined 511 Mb genomic sequence of the Asian corn
borer, Ostrinia furnacalis, a congener of O. nubilalis, and isolated BAC
and fosmid clones that were expected to localize in candidate regions for
the boundaries using these sequences. Combined with FISH and genetic
analysis, we narrowed down the candidate regions to 40 kb-1.5 Mb, in
strong agreement with a previous estimate based on the genome of a
butterfly, Melitaea cinxia. The significant difference in the lengths of
the candidate regions where no functional genes were observed may reflect
the evolutionary time after fusion events.
O. furnacalis scaffold
Japan
Fukushima