10.5061/DRYAD.PM71K02
Raquin, Vincent
University of Lyon System
Henri, Hélène
University of Lyon System
Vallat, Marine
University of Lyon System
Leulier, François
University of Lyon System
Gibert, Patricia
University of Lyon System
Kremer, Natacha
University of Lyon System
Data from: Development of a PCR-RFLP assay to identify Drosophila
melanogaster among field-collected larvae
Dryad
dataset
2019
Fruit fly larvae
Phortica
taxonomic identification
PCR-RFLP
Drosophila immigrans
field samples
Drosophila suzukii
2019-07-25T00:00:00Z
2019-07-25T00:00:00Z
en
https://doi.org/10.1002/ece3.4453
28004178 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The fruit fly Drosophila melanogaster is a model organism to study several
aspects of metazoan biology. Most of the work has been conducted in adult
fruit flies, including laboratory and field-derived specimens, but
Drosophila melanogaster larvae recently became a valuable model to better
understand animal physiology, development or host-microbe interactions.
While adult flies can be easily assigned to a given Drosophila species
based on morphological characteristics, such visual identification is more
intricate at the larval stage. This could explain the limited number of
studies focusing on larvae, especially field-derived samples. Here, we
developed a Polymerase Chain Reaction-Restriction Fragment Length
Polymorphism (PCR-RFLP) assay that discriminates D. melanogaster from
other ecologically relevant Drosophila species at the larval stage. The
method, which targets the cytochrome oxidase I (COI) gene, was validated
using laboratory-derived larvae from seven D. melanogaster populations
originating from different geographic areas as well as six Drosophila
species. We further validated this PCR-RFLP assay in a natural context, by
identifying wild larvae collected in two locations in France. Notably,
among all PCR-RFLP profiles that matched the D. melanogaster species, 100%
were correctly identified, as confirmed by COI sequencing. In summary, our
work provides a rapid, simple and accurate molecular tool to identify D.
melanogaster from field-collected larvae.
Development of a PCR-RFLP assay to identify Drosophila melanogaster among
field-collected larvaeSanger sequencing of COI PCR fragment from wild
Drosophila larvaeDeposit.zip
France