10.5061/DRYAD.PG4F4QRJ9
Paerl, Ryan
0000-0003-3980-8181
North Carolina State University
Claudio, Iliana
North Carolina State University
Shields, Michael
Texas A&M University
Bianchi, Thomas
University of Florida
Osburn, Christopher
North Carolina State University
Dityrosine formation via reactive oxygen consumption yields increasingly
recalcitrant humic-like fluorescent organic matter in the ocean
Dryad
dataset
2019
DOM
FDOM
dityrosine
Tyrosine
reactive oxygen species (ROS)
National Science Foundation
https://ror.org/021nxhr62
OCE 1459406
National Science Foundation
https://ror.org/021nxhr62
OCE 1459294
2020-02-25T00:00:00Z
2020-02-25T00:00:00Z
en
https://doi.org/10.5061/dryad.pg4f4qrj9
50237 bytes
5
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Marine dissolved organic matter (DOM) is a massive elemental pool on Earth
and is thought to consist of a chemically complex mixture of molecules.
Part of marine DOM is fluorescent (FDOM) and includes humic-like
compounds. The chemical composition of, and biochemical pathways that
yield, autochthonous humic-like FDOM in the ocean is largely unknown.
Inspired by medical and biochemical research detailing the formation of
colored and fluorescent dityrosine via peroxidase mediated reactions, we
used fluorometry and spectroscopy and found dityrosine exhibits humic-like
fluorescence similar to that of marine FDOM. Investigating its
recalcitrance, we conducted short-term nutrient-addition and light
exposure experiments with dityrosine and found it is resistant to
respiration or assimilation by marine microbial communities, yet readily
photodegraded. The formation and characteristics of dityrosine newly
points to polymerization via reactive oxygen quenching, under aphotic and
putatively photic conditions, as an explanation for autochthonous,
humic-like, (semi-)recalcitrant DOM in the ocean.