10.5061/DRYAD.PG034
Baillie, Shauna M.
Dalhousie University
Hemstock, Riley R.
Dalhousie University
Muir, Andrew M.
Great Lakes Fishery Commission
Krueger, Charles C.
Michigan State University
Bentzen, Paul
Dalhousie University
Data from: Small-scale intraspecific patterns of adaptive immunogenetic
polymorphisms and neutral variation in Lake Superior lake trout
Dryad
dataset
2018
MHC Class IIB
2018-05-02T00:00:00Z
2018-05-02T00:00:00Z
en
https://doi.org/10.1007/s00251-017-0996-4
37746 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Many fishes express high levels of intraspecific variability, often linked
to resource partitioning. Several studies show that a species’
evolutionary trajectory of adaptive divergence can undergo reversals
caused by changes in its environment. Such a reversal in neutral genetic
and morphological variation among lake trout Salvelinus namaycush
ecomorphs appears to be underway in Lake Superior. However, a water depth
gradient in neutral genetic divergence was found to be associated with
intraspecific diversity in the lake. To investigate patterns of adaptive
immunogenetic variation among lake trout ecomorphs, we used Illumina
high-throughput sequencing. The population’s genetic structure of the
major histocompatibility complex (MHC Class IIβ exon 2) and 18
microsatellite loci were compared to disentangle neutral and selective
processes at a small geographic scale. Both MHC and microsatellite
variation were partitioned more by water depth stratum than by ecomorph.
Several metrics showed strong clustering by water depth in MHC alleles,
but not microsatellites. We report a 75% increase in the number of MHC
alleles shared between the predominant shallow and deep water ecomorphs
since a previous lake trout MHC study at the same locale (c. 1990s data).
This result is consistent with the reverse speciation hypothesis, although
adaptive MHC polymorphisms persist along an ecological gradient. Finally,
results suggested that the lake trout have multiple copies of the MHC II
locus consistent with a historic genomic duplication event. Our findings
indicated that conservation approaches for this species could focus on
managing various ecological habitats by depth, in addition to regulating
the fisheries specific to ecomorphs.
Genepop_MHC_DominantBinary_DryadGenepop_Msats_Dryad
USA
Lake Superior
Michigan