10.5061/DRYAD.PF012V0
Pires, Ricardo H.
University of Greifswald
Shree, Nithya
University of Greifswald
Manu, Emmanuel
University of Greifswald
Guzniczak, Ewa
Heriot-Watt University
Otto, Oliver
University of Greifswald
Data from: Cardiomyocyte mechanodynamics under conditions of actin remodelling
Dryad
dataset
2019
Cardiomyocytes
real-time deformability cytometry
contractility
Cell mechanics
2019-10-16T00:00:00Z
2019-10-16T00:00:00Z
en
https://doi.org/10.1098/rstb.2019.0081
15862666 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The mechanical performance of cardiomyocytes is an important indicator of
their maturation state and of primary importance for the development of
therapies based on cardiac stem-cells. As the mechanical analysis of
adherent cells at high-throughput remains challenging, we explore the
applicability of real-time deformability cytometry (RT-DC) to probe
cardiomyocytes in suspension. RT-DC is a microfluidic technology allowing
for real-time mechanical analysis of thousands of cells with a throughput
exceeding 1,000 cells per second. For cardiomyocytes derived from human
induced pluripotent stem cells, we determined a Young’s modulus of
1.250.08 kPa which is in close range to previous reports. Upon
challenging the cytoskeleton with cytochalasin D (CytoD) to induce
filamentous actin depolymerization, we distinguish three different regimes
in cellular elasticity. Transitions are observed below 10 nM and above 103
nM and are characterized by a decrease in the Young’s modulus. These
regimes can be linked to cytoskeletal and sarcomeric actin contributions
by cardiomyocyte contractility measurements at varying CytoD
concentrations, where we observe a significant reduction in pulse duration
only above 103 nM while no change is found for compound exposure at lower
concentrations. Comparing our results to mechanical cell measurements
using atomic force microscopy we demonstrate for the first time the
feasibility of using a microfluidic technique to measure mechanical
properties of large samples of adherent cells while linking our results to
the composition of the cytoskeletal network.
Figure1 RT-DCThis file contains the data of mechanical phenotype for
cardiomyocytes measured by RT_DCFigure1_RT_DC_M1_data.tdmsFigure 1
Fluorescence controlThis file contains the unlabelled control.control 3 -
M1.fcsFigure 1 Fluorescence DNAThis file contains the DNA stainsample 1uL
- M1.fcsFigure 2 RT-DC DMSO 1 controlThis file contains the DMSO 1 control
of the RT-DC measurementFigure2_RT_DC_DMSO1_M1_data.tdmsFigure 2 RT-DC
DMSO 2 controlDMSO control 2 for RT-DC
measurementsFigure2_RT_DC_DMSO2_M1_data.tdmsFigure 2 RT-DC CytoD 1
0.01uMRT-DC CytoD 1 0.01uM
dataFigure2_RT_DC_CytoD1_0_01_M1_data.tdmsFigure 2 RT-DC CytoD 2 0.01
uMRT-DC data for CytoD repeat 2 0.01 uM
measurementFigure2_RT_DC_CytoD2_0_01_M1_data.tdmsFigure 2 RT-DC CytoD 1
0.1uMRT-DC data from CytoD 1 0.1uM
measurementFigure2_RT_DC_CytoD1_0_1_M1_data.tdmsFigure 2 RT-DC CytoD 2
0.1uMRT-DC data from CytoD 2 0.1uM
measurementFigure2_RT_DC_CytoD2_0_1_M1_data.tdmsFigure 2 RT-DC CytoD 1
1uMRT-DC data from CytoD 1 1uM
measurementFigure2_RT_DC_CytoD1_1_M1_data.tdmsFigure 2 RT-DC CytoD 2
1uMRT-DC data from CytoD 2 1uM
measurementFigure2_RT_DC_CytoD2_1_M1_data.tdmsFigure 2 RT-DC CytoD 1
10uMRT-DC data from CytoD 1 10uM
measurementFigure2_RT_DC_CytoD1_10_M1_data.tdmsFigure 2 RT-DC CytoD 2
10uMRT-DC data from CytoD 2 10uM
measurementFigure2_RT_DC_CytoD2_10_M1_data.tdmsFigure 2 AFM
HistogramFigure 2 AFM data histogramFigure2_AFM_Histogram.datFigure 2 AFM
comparisonFigure 2 data AFM comparisonFigure2_AFM_Comparison.csvFigure 2
contractilityFigure 2 data cardiomyocyte
contractilityFigure2_Contractility_Data.txt