10.5061/DRYAD.PD28PM7N
Dupont, Sebastien
Procédés Alimentaires et Microbiologiques
Lemetais, Guillaume
Procédés Alimentaires et Microbiologiques
Ferreira, Thierry
French National Centre for Scientific Research
Cayot, Philippe
Procédés Alimentaires et Microbiologiques
Gervais, Patrick
Procédés Alimentaires et Microbiologiques
Beney, Laurent
Procédés Alimentaires et Microbiologiques
Data from: Ergosterol biosynthesis: a fungal pathway for life on land?
Dryad
dataset
2012
2012-03-27T18:46:43Z
2012-03-27T18:46:43Z
en
https://doi.org/10.1111/j.1558-5646.2012.01667.x
29696 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Sterols, essential lipids of most eukaryotic cells, ensure important
structural and signaling functions. The selection pressure which has led
to different dominant sterols in the three eukaryotic kingdoms remains
unknown. Here we investigated the influence of the progression in the
different steps of the ergosterol biosynthetic pathway (EBP) on the yeast
resistance to transitions from aqueous to aerial media, typical
perturbations of the higher fungi habitats. Five mutants of the EBP
(ergΔ), accumulating different sterol intermediates in the EBP, and the WT
strain were exposed to drying under atmospheric air or nitrogen and
wetting. Results show that the progression in the EBP parallels an
increase in the yeast resistance to air-drying with a maximal survival
rate for the WT strain. When drying/wetting was performed under nitrogen,
yeast survival was higher, particularly for the earlier mutants of the
EBP. Thus, ergosterol, through its protective role against mechanical and
oxidative stress, might have been selected by the pressure induced by
drying/wetting cycles occurring in the fungi habitats. These results
support the Bloch hypothesis, which postulates that the properties of
sterols are gradually optimized for function along the biosynthetic
pathway and provide a response to the enduring question “why ergosterol in
fungi?”.
DataData concerning the survival of the different yeast strains after
drying treatments and yeast sterol analysis