10.5061/DRYAD.P7P7P
Holding, Matthew L.
The Ohio State University
Biardi, James E.
Fairfield University
Gibbs, H. Lisle
The Ohio State University
Data from: Coevolution of venom function and venom resistance in a
rattlesnake predator and its squirrel prey
Dryad
dataset
2016
Crotalus oreganus
venom
molecular variation
Otospermophilus beecheyi
Coevolution
2016-04-06T13:46:19Z
2016-04-06T13:46:19Z
en
https://doi.org/10.1098/rspb.2015.2841
129566 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Measuring local adaptation can provide insights into how coevolution
occurs between predators and prey. Specifically, theory predicts that
local adaptation in functionally-matched traits of predators and prey will
not be detected when coevolution is governed by escalating arms races,
whereas it will be present when coevolution occurs through an alternate
mechanism of phenotype matching. Here, we analyze local adaptation in
venom activity and prey resistance across 12 populations of Northern
Pacific rattlesnakes and California ground squirrels, an interaction that
has often been described as an arms race. Assays of venom function and
squirrel resistance show substantial geographic variation (influenced by
site elevation) in both venom metalloproteinase activity and resistance
factor effectiveness. We demonstrate local adaptation in the effectiveness
of rattlesnake venom to overcoming present squirrel resistance, suggesting
that phenotype matching plays a role in the coevolution of these molecular
traits. Further, the predator was the locally adapted antagonist in this
interaction, arguing that rattlesnakes are evolutionarily ahead of their
squirrel prey. Phenotype matching needs to be considered as an important
mechanism influencing coevolution between venomous animals and resistant
prey.
Venom metalloproteinase activity and Squirrel InhibitionThis file includes
1440 rows of snake venom metalloproteinase activity data, where each row
is defined by a unique combination of of rattlesnake (Crotalus oreganus)
and a ground squirrel (Otospermophilus beecheyi). Columns include animal
identification numbers, animal collection site, site elevation, geographic
distance between snake and squirrel pairings, baseline metalloproteinase
activity of each snake's venom, and the metalloproteinase activity
achieved in the presence of ground squirrel blood serum, which contains
venom inhibitors. This dataset was used to test for local adaptation in
the interactions between rattlesnake predators and squirrel prey in the
reference manuscript.SVMP_Dryad.xlsx
California