10.5061/DRYAD.P72P0
Österman, Janina
University of Helsinki
Chizhevskaja, Elena P.
University of Helsinki
Andronov, Evgeny E.
All-Russian Research Institute of Agricultural Microbiology
Fewer, David P.
University of Helsinki
Terefework, Zewdu
University of Helsinki
Roumiantseva, Marina L.
All-Russian Research Institute of Agricultural Microbiology
Onichtchouk, Olga P.
All-Russian Research Institute of Agricultural Microbiology
Dresler-Nurmi, Aneta
University of Helsinki
Simarov, Boris V.
All-Russian Research Institute of Agricultural Microbiology
Dzyubenko, Nikolay I.
Department of Forage Sciences, N. I. Vavilov Russia Research Institute
of Plant Genetic Resources, St. Petersburg 190000, Russia
Lindstrom, Kristina
Data from: Galega orientalis is more diverse than Galega officinalis in
Caucasus – whole-genome AFLP analysis and phylogenetics of
symbiosis-related genes
Dryad
dataset
2011
Galega officinalis
Rhizobium galegae
Galega orientalis
Species interactions
Coevolution
2011-08-10T21:16:38Z
2011-08-10T21:16:38Z
en
https://doi.org/10.1111/j.1365-294X.2011.05291.x
807031 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Legume plants can obtain combined nitrogen for their growth in an
efficient way through symbiosis with specific bacteria. The symbiosis
between Rhizobium galegae and its host plant Galega is an interesting case
where the plant species G. orientalis and G. officinalis form effective,
nitrogen fixing, symbioses only with the appropriate rhizobial
counterpart, R. galegae bv. orientalis and R. galegae bv. officinalis
respectively. There is plenty of information available on the symbiotic
properties of nitrogen fixing rhizobia, while more information is needed
on the properties of the host plants. The Caucasus region in Eurasia has
been identified as the gene centre (centre of origin) of G. orientalis,
although both G. orientalis and G. officinalis can be found in this
region. In this study, the diversity of these two Galega species in
Caucasus was investigated to test the hypothesis that in this region G.
orientalis is more diverse than G. officinalis. The amplified fragment
length polymorphism (AFLP) fingerprinting performed here showed that the
populations of G. orientalis and R. galegae bv. orientalis are more
diverse than those of G. officinalis and R. galegae bv. officinalis
respectively. These results are consistent with the centre of origin
status of Caucasus for G. orientalis. Phylogenies of the symbiosis-related
plant genes NORK and Nfr5 were congruent with the AFLP result from a
diversity point of view. Finally, the results of this work indicate that
the NORK and Nfr5 genes of Galega follow the same evolutionary pattern as
conserved plant genes.
Plant AFLP patternsThis file contains the normalized AFLP patterns of the
Galega accessions from Caucasus, created with BioNumerics. These patterns
functioned as the basis for the curve-based analysis, used to construct
the similarity matrix.Plant_AFLP.pdfRhizobium AFLP patternsThis file
contains the normalized gel picture of the AFLP patterns for the Rhizobium
galegae strains representing the R. galegae population in Caucasus. The
file was created with BioNumerics and used in the curve-based analysis to
construct the similarity matrix of the rhizobial AFLP
analysis.Rhizobium_AFLP.pdfGalega AFLP similarity matrixThe similarity
matrix obtained from the BioNumerics analysis of the Galega AFLP
data.Galega_AFLP_similarity_matrix.txtRhizobium AFLP similarity matrixThe
similarity matrix obtained from the BioNumerics analysis of the Rhizobium
galegae AFLP data.Rhizobium_AFLP_similarity_matrix.txtNORK
neighbor-joining tree of complete datasetThe neighbor-joining tree used
for the codeml analyses of the NORK gene. The tree was constructed in MEGA
4, with the substitution model of Tamura-Nei + gamma 1.0 and complete
deletion of gaps. Bootstrap values > 70 % are
shown.NORK_NJ_tree_All_Galegas.pdfNfr5 neighbor-joining tree of complete
datasetThe neighbor-joining tree used for the codeml analyses of the Nfr5
gene. The tree was constructed in MEGA 4, with the substitution model of
Tamura-Nei + gamma 1.0 and pairwise deletion of gaps and missing data.
Bootstrap values > 70 % are shown.Nfr5_NJ_tree_All_Galegas.pdf