10.5061/DRYAD.P100G
Jiang, Wei
Shanghai Institutes for Biological Sciences
Institute of Biochemistry
Tang, Jing-Jie
Shanghai Institutes for Biological Sciences
Institute of Biochemistry
Miao, Hong-Hua
Shanghai Institutes for Biological Sciences
Institute of Biochemistry
Qu, Yu-Xiu
Shanghai Institutes for Biological Sciences
Institute of Biochemistry
Qin, Jie
Shanghai Institutes for Biological Sciences
Institute of Biochemistry
Xu, Jie
Shanghai Institutes for Biological Sciences
Institute of Biochemistry
Yang, Jinbo
Lanzhou University
Li, Bo-Liang
Shanghai Institutes for Biological Sciences
Institute of Biochemistry
Song, Bao-Liang
Shanghai Institutes for Biological Sciences
Institute of Biochemistry
Wuhan University
Data from: Forward genetic screening for regulators involved in
cholesterol synthesis using validation-based insertional mutagenesis
Dryad
dataset
2014
somatic cell genetics
SREBP
forward genetic screen
HMG-CoA reductase
VBIM
Cholesterol
SCAP
2014-12-02T17:22:09Z
2014-12-02T17:22:09Z
en
https://doi.org/10.1371/journal.pone.0112632
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Somatic cell genetics is a powerful approach for unraveling the regulatory
mechanism of cholesterol metabolism. However, it is difficult to identify
the mutant gene(s) due to cells are usually mutagenized chemically or
physically. To identify important genes controlling cholesterol
biosynthesis, an unbiased forward genetics approach named validation-based
insertional mutagenesis (VBIM) system was used to isolate and characterize
the 25-hydroxycholesterol (25-HC)-resistant and SR-12813-resisitant
mutants. Here we report that five mutant cell lines were isolated. Among
which, four sterol-resistant mutants either contain a truncated
NH2-terminal domain of sterol regulatory element-binding protein (SREBP)-2
terminating at amino acids (aa) 400, or harbor an overexpressed SREBP
cleavage-activating protein (SCAP). Besides, one SR-12813 resistant mutant
was identified to contain a truncated COOH-terminal catalytic domain of
3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase). This
study demonstrates that the VBIM system can be a powerful tool to screen
novel regulatory genes in cholesterol biosynthesis.
SL-4 genome sequenceGene locus of SCAP in SL-4 mutant. This sequence is
related to Fig.3SL-4 genome seq.docx