10.5061/DRYAD.NZS7H44QP
He, Shulin
0000-0003-4817-5646
Freie Universität Berlin
Freie Universität Berlin
Sieksmeyer, Thorben
Freie Universität Berlin
Freie Universität Berlin
Che, Yanli
Southwest University
China Society of Plant Protection
Mora, M. Alejandra Esparza
Freie Universität Berlin
Freie Universität Berlin
Banasiak, Ronald
Federal Institute For Materials Research and Testing
Federal Institute For Materials Research and Testing
Harrison, Mark C.
University of Münster
Stiblik, Petr
0000-0001-6141-5603
Czech University of Life Sciences Prague
Czech University of Life Sciences Prague
Jan, Šobotník
Czech University of Life Sciences Prague
Wang, Zongqing
Southwest University
China Society of Plant Protection
Johnston, Paul
Freie Universität Berlin
McMahon, Dino P.
0000-0003-1119-5299
Freie Universität Berlin
Freie Universität Berlin
Šobotník, Jan
Czech University of Life Sciences Prague
Johnston, Paul R.
0000-0002-8651-4488
Freie Universität Berlin
Evidence for reduced immune gene diversity and activity during the
evolution of termites
Dryad
dataset
2020
social insect
Subsocial
cockroach
major transition
contraction
expansion
2021-07-27T00:00:00Z
2021-07-27T00:00:00Z
en
https://doi.org/10.1098/rspb.2020.3168
2136790653 bytes
5
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
This dataset contains data from a termite immunity related study described
in the paper: “He Shulin, Sieksmeyer Thorben, Che Yanli, Mora M. Alejandra
Esparza, Stiblik Petr, Banasiak Ronald, Harrison Mark C., Šobotník Jan,
Wang Zongqing, Johnston Paul R. and McMahon Dino P. 2021Evidence for
reduced immune gene diversity and activity during the evolution of
termitesProc. R. Soc.
B.288:20203168.http://doi.org/10.1098/rspb.2020.3168”. The study
investigates the evolution of termite molecular immune system: evolution
of immune gene family along a constructed phylogeny, different individual
immune response between three termite castes, a subsocial cockroach and a
non-social cockroach, the caste specific expression of immune genes,
different social immune response between a social termite species and a
non-social cockroach species. In the first experiment, we de novo
sequenced 18 cockroach and termite species, spanning the full spectrum of
solitary and social lifestyles, including two solitary cockroach species,
two species of subsocial Cryptocercus wood-feeding cockroaches and 14
termite species. We exploited a transcriptomic approach to compare the
immune gene repertoire of these sequenced species. In the second
experiment, we compared individual immune responses in a solitary
cockroach, B. orientalis, a subsocial wood-feeding roach, Cryptocercus
meridianus, and each caste of a social termite, Neotermes castaneus,
following direct injection with heat-killed microbes. In the third
experiment, we explored total gene expression differences between castes
without immune challenge. In the fourth experiment, we studied gene
expression changes in each caste of N. castaneus following colony exposure
to immune-challenged nestmates, and compared these with gene expression
changes in the solitary cockroach, B. orientalis, following group exposure
to immune-challenged conspecifics. Main results of the experiments are
that (1) immune gene families show contractions and expansions during
temite evolution; (2) compared with cockroaches, termites showed weak
individual immune response; (3) termites have caste-specific constitutive
immunity; (4) Compared with cockroach, termite showed a stronger gene
expression changes in response to a social immune challenge.
The dataset was collected for a study of termite immunity evolution at
Freie Universität Berlin and BAM Federal Institute for Materials Research
and Testing. It has been processed to produce a MS accepted for
publication in Proceedings of the Royal Society B.
The readme file contains an explanation of the files in the dataset.
Information on how the data were produced can be found in the associated
manuscript referenced above and related github repository.