10.5061/DRYAD.NT615
Banu, Sayera
Pholwat, Suporn
University of Virginia
Foongladda, Suporn
Mahidol University
Chinli, Rattapha
Mahidol University
Boonlert, Duangjai
Mahidol University
Ferdous, Sara Sabrina
Rahman, S.M. Mazidur
Rahman, Arfatur
Ahmed, Shahriar
Houpt, Eric R.
University of Virginia
Data from: Performance of TaqMan Array Card to detect TB drug resistance
on direct specimens
Dryad
dataset
2017
TB drug resistance
Mycobacterium tuberculosis
Direct specimen
TaqMan array card
2017-05-15T15:24:03Z
2017-05-15T15:24:03Z
en
https://doi.org/10.1371/journal.pone.0177167
415605 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Culture based phenotypic drug susceptibility testing (DST) for
Mycobacterium tuberculosis (TB) is time consuming therefore rapid
genotypic methods are increasingly being utilized. We previously developed
and evaluated on TB isolates a rapid genotypic TaqMan array card (TAC)
that detects mutations in several resistance-associated genes using dozens
of primer pairs, probes, and high resolution melt analysis, with
>96% accuracy versus Sanger sequencing. In this study we examined
the performance of TAC on sputum, comparing results between 71 paired
sputum and multi drug resistant TB isolates. We also adapted the TAC to
include wild-type probes and broadened coverage for rpoB and gyrA
mutations. TAC was 89% successful at detecting wild-type or mutations
within inhA, katG, rpoB, eis, gyrA, rplC, and pncA on smear positive sputa
and 33% successful on smear negative sputa. The overall accuracy of these
detections as compared to the TAC results of the paired isolate was 95% ±
7 (average sensitivity 98% ± 3; specificity 92% ± 14). Accuracy of sputum
TAC results versus phenotypic DST for isoniazid, rifampin,
amikacin/kanamycin, ofloxacin/moxifloxacin, and pyrazinamide was 87% ± 11.
This was similar to that of the paired isolate TAC results (accuracy 90% ±
12) and inaccuracies primarily reflected intrinsic genotypic-phenotypic
discordance. The TAC is a rapid, modular, comprehensive, and accurate TB
DST for the major first and second line TB drugs on smear positive sputum.
Raw data
Bangladesh
Thailand