10.5061/DRYAD.NC3G27S
Scott, Peter A.
University of Alabama, Tuscaloosa
Glenn, Travis C.
University of Georgia
Rissler, Leslie J.
University of Alabama, Tuscaloosa
Data from: Formation of a recent hybrid zone offers insight to the
geographic puzzle and maintenance of species boundaries in musk turtles
Dryad
dataset
2018
Anthropocene
Bayesian Genomic Cline
Sternotherus
Anthropogenic Habitat Change
3RAD
Sternotherus depressus
Parapatric Species Boundaries
Sternotherus peltifer
2018-12-19T15:44:18Z
2018-12-19T15:44:18Z
en
https://doi.org/10.1111/mec.14983
13953633 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Speciation is the result of an accumulation of reproductive barriers
between populations, pinpointing these factors is often difficult.
However, hybrid zones can form when these barriers are not complete,
especially when lineages come into contact in intermediate or modified
habitats. We examine a hybrid zone between two closely related riverine
turtle species, Sternotherus depressus and S. peltifer, and use ddRAD
sequencing to to understand how this hybrid zone formed and elucidate
genomic patterns of reproductive isolation. First the geographic extent
and timing of the hybrid zone formation is established to provide context
for understanding the role of extrinsic and intrinsic reproductive
isolating mechanisms in this system. Then, the strength of selection on
taxon-specific contributions to maintenance of the hybrid zone is inferred
using a Bayesian genomic cline model. These analyses find a role for
selection inhibiting introgression in some genomic regions at one end of
the hybrid zone and promoting introgression in many loci at the other.
When selective pressures necessary to generate outliers to the genomic
cline are considered with the geographic and temporal context of this
hybrid zone we conclude that habitat-specific selection likely limits
introgression from S. depressus to S. peltifer in the direction of river
flow. However, selection is mediating rapid, unidirectional introgression
from S. peltifer to S. depressus which is likely facilitated by
anthropogenic habitat alteration. These findings indicate a potentially
imminent threat of population-level genomic extinction for an already
imperiled species due to ongoing human-caused habitat alteration.
Structure input fileThe input file to recreate the structure
analysisSpSd_8deep90SH40.strA folder of input files to run the partitioned
D-statistic in pyRADA folder containing the .loci file with genomic data
and three parameter files to recreate partitioned D-test using the program
pyRAD.partitioned_D-test.tar.gzNewHybrids input fileAn input file to run
NewHybrids on a subsample of 500 random SNPs for the S. depressus/peltifer
hybrid zone.NewHybrids_SpSd_500.txtBGC inputAllele frequency data for pure
S. depressus, pure S. peltifer, and the hybrid zone for input for the
Bayesian Genomic Clines program.BGC.tar.gzD-foil input/outputFolders
containing input files for D-foil, a shell script to loop over all input
files and the output from D-foil analyses. These are broken up into three
imput folders to represent the comparisons among the three water sheds in
the distribution of S. depressus.D-foil.tar.gz
eastern North America
United States