10.5061/DRYAD.N8147
Burns, Mercedes
San Diego State University
University of Maryland, College Park
Hedin, Marshal
San Diego State University
Tsurusaki, Nobuo
Tottori University
Data from: Population genomics and geographical parthenogenesis in
Japanese harvestmen (Opiliones, Sclerosomatidae, Leiobunum)
Dryad
dataset
2018
Leiobunum manubriatum
Leiobunum globosum
National Science Foundation
https://ror.org/021nxhr62
DBI-1401110
2018-10-16T00:00:00Z
2018-10-16T00:00:00Z
en
https://doi.org/10.1002/ece3.3605
2020364 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Naturally-occurring population variation in reproductive mode presents an
opportunity for researchers to test hypotheses regarding the evolution of
sex. Such populations frequently assume a geographical pattern, in which
parthenogenesis-dominated populations are widely dispersed, with narrowly
distributed sexual populations. We evaluate the geographic distribution of
genomic signatures associated with parthenogenesis using nuclear and
mitochondrial DNA sequence data from two Japanese harvestman sister taxa,
Leiobunum manubriatum and L. globosum. Asexual reproduction is putatively
facultative in these species, and female-biased localities are common in
habitat margins. Past karyotypic and current cytometric work indicates L.
globosum is entirely tetraploid, while L. manubriatum may be either
diploid or tetraploid. We estimated species phylogeny, genetic
differentiation, diversity, and mitonuclear discordance in females
collected across the species range in order to identify range expansion
towards marginal habitat, potential for hybrid origin, and persistence of
asexual lineages. Our results point to northward expansion of a tetraploid
ancestor of L. manubriatum and L. globosum, coupled with support for
greater male gene flow in southern L. manubriatum localities. Specimens
from localities in the Tohoku and Hokkaido regions were indistinct,
particularly those of L. globosum, potentially due to little mitochondrial
differentiation or haplotypic variation. Although L. manubriatum overlaps
with L. globosum across its entire range, L. globosum was reconstructed as
monophyletic with strong support using mtDNA, and marginal support with
nuclear loci. Ultimately, we find evidence for continued sexual
reproduction in both species, and describe opportunities to clarify the
rate and mechanism of parthenogenesis.
GMgbs2ploidyR script for application of gbs2ploidy packageDAPC and
statistics scriptR script to compute population statistics and DAPC
analysesDAPCstats.RBEAST *.xml files*.xml-formatted files for L. globosum,
L. manubriatum, and all specimensBEASTfiles.zipFiles for DAPC and
population statisticsFolder of *.phy, STRUCTURE and tab delimited files
for F-statistics calculation and DAPC analyses in RRfiles.zipgbs2ploidy
filesEdited heterozygous allele depth files and FACS-assigned ploidy for
all specimens to use in gbs2ploidy analyses in R.ploidyfiles.zip
Japan