10.5061/DRYAD.N5095
Bhat, Ramray
Lawrence Berkeley National Laboratory
Chakraborty, Mahul
University of California, Irvine
Glimm, Tilmann
University of Washington
Stewart, Thomas A.
Yale University
Newman, Stuart A.
New York Medical College
Data from: Deep phylogenomics of a tandem-repeat galectin regulating
appendicular skeletal pattern formation
Dryad
dataset
2016
galectin
Mathematical modeling
Pattern formation
2016-07-26T17:33:29Z
2016-07-26T17:33:29Z
en
https://doi.org/10.1186/s12862-016-0729-6
60552 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: A multiscale network of two galectins Galectin-1 (Gal-1) and
Galectin-8 (Gal-8) patterns the avian limb skeleton. Among vertebrates
with paired appendages, chondrichthyan fins typically have one or more
cartilage plates and many repeating parallel endoskeletal elements,
actinopterygian fins have more varied patterns of nodules, bars and
plates, while tetrapod limbs exhibit tandem arrays of few, proximodistally
increasing numbers of elements. We applied a comparative genomic and
protein evolution approach to understand the origin of the galectin
patterning network. Having previously observed a phylogenetic constraint
on Gal-1 structure across vertebrates, we asked whether evolutionary
changes of Gal-8 could have critically contributed to the origin of the
tetrapod pattern. Results: Translocations, duplications, and losses of
Gal-8 genes in Actinopterygii established them in different genomic
locations from those that the Sarcopterygii (including the tetrapods)
share with chondrichthyans. The sarcopterygian Gal-8 genes acquired a
potentially regulatory non-coding motif and underwent purifying selection.
The actinopterygian Gal-8 genes, in contrast, did not acquire the
non-coding motif and underwent positive selection. Conclusion: These
observations interpreted through the lens of a reaction-diffusion-adhesion
model based on avian experimental findings can account for the distinct
endoskeletal patterns of cartilaginous, ray-finned, and lobe-finned
fishes, and the stereotypical limb skeletons of tetrapods.
BMC Evolutionary Biology EVOB-D-16-00044R3Protein sequences, CDS
nucleotide sequences and promoter sequences used in the analyses of the
paper