10.5061/DRYAD.N34H180
Zhang, Yuanyuan
Wageningen University & Research
Bouwmeester, Harro J.
University of Amsterdam
Kappers, Iris F.
0000-0003-3349-3473
Wageningen University & Research
Data from: Combined transcriptome and metabolome analysis identifies
defence responses in spider-mite infested pepper
Dryad
dataset
2019
Tetranychus urticae
plant-arthropod interactions
Capsicum annuum
JA/SA crosstalk
specialized metabolites
transcriptional changes
two-spotted spider mites
2019-10-11T00:00:00Z
2019-10-11T00:00:00Z
en
https://doi.org/10.1093/jxb/erz422
4406069 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Plants regulate responses towards herbivory through fine-tuning of
defence-related hormone production, expression of defence genes and
production of secondary metabolites. Jasmonic acid (JA) plays a key role
in plant-herbivorous arthropod interactions. To understand how pepper
responds to herbivory, leaf transcriptomes and metabolomes of two
genotypes different in their susceptibility to spider mites, were studied.
Mites induced both JA and salicylic acid (SA) signalling. However, mite
infestation and exogenous JA resulted in distinct transcriptome profiles.
Compared with JA, mites induced less differentially expressed genes
involved in metabolic processes (except for genes involved in the
phenylpropanoid pathway) and lipid metabolic processes. Furthermore,
pathogen-related defence responses including WRKY transcription factors,
were stronger induced upon mite infestation, likely as result of induced
SA signalling. Untargeted analysis of secondary metabolites confirmed that
JA treatment induced larger changes in metabolism than spider-mite
infestation, resulting in a higher terpenoid and flavonoid production. The
more resistant genotype exhibited a larger increase in endogenous JA and
volatile and non-volatile secondary metabolites upon infestation, which
could explain its stronger defence. Reasoning that in JA-SA antagonizing
crosstalk, SA-defences are prioritized over JA-defences, we hypothesize
that lack of SA-mediated repression of JA-induced defences could result in
gain-of-resistance towards spider mites in pepper.
Genes and metabolites associated with spider-mite induced responses in
pepper Gene transcripts, hormone and metabolite analysis