10.5061/DRYAD.N2645
Carroll, Sean Michael
Harvard University
Lee, Ming-Chun
University of Hong Kong
Harvard University
Marx, Christopher James
Harvard University
Data from: Sign epistasis limits evolutionary trade-offs at the confluence
of single- and multi-carbon metabolism in Methylobacterium extorquens AM1
Dryad
dataset
2013
trade-offs
Epistasis
Selection - Experimental
2013-10-08T14:24:33Z
2013-10-08T14:24:33Z
en
https://doi.org/10.1111/evo.12301
1130531 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Adaptation of one set of traits is often accompanied by attenuation of
traits important in other selective environments, leading to fitness
trade-offs. The mechanisms that either promote or prevent the emergence of
trade-offs remain largely unknown, and are difficult to discern in most
systems. Here, we investigate the basis of trade-offs that emerged during
experimental evolution of Methylobacterium extorquens AM1 to distinct
growth substrates. After 1500 generations of adaptation to a multi-carbon
substrate, succinate (S), many lineages had lost the ability to use
one-carbon compounds such as methanol (M), generating a mixture of M+ and
M− evolved phenotypes. We show that trade-offs in M− strains consistently
arise via antagonistic pleiotropy through recurrent selection for
loss-of-function mutations to ftfL (formate-tetrahydrofolate ligase),
which improved growth on S while simultaneously eliminating growth on M.
But if loss of FtfL was beneficial, why were M trade-offs not found in all
populations? We discovered that eliminating FtfL was not universally
beneficial on S, as it was neutral or even deleterious in certain evolved
lineages that remained M+. This suggests that sign epistasis with earlier
arising mutations prevented the emergence of mutations that drove
trade-offs through antagonistic pleiotropy, limiting the evolution of
metabolic specialists in some populations.
Data_CombinedData files for the article: Sign epistasis limits
evolutionary trade-offs at the confluence of single- and multi-carbon
metabolism in Methylobacterium extorquens AM1. Sean Michael Carroll*,
Ming-Chun Lee*, Christopher J. Marx *These authors contributed equally to
this work Accepted for publication in Evolution. October, 2013