10.5061/DRYAD.MSBCC2FVR
Deveau, Ciana
0000-0001-9601-6629
National Institutes of Health
Jiao, Xiaodong
,
Suzuki, Sachihiro
,
Krishnakumar, Asha
,
Yoshimatsu, Takeshi
,
Hejtmancik, J Fielding
,
Nelson, Ralph
,
Data from: Thyroid hormone receptor beta mutations alter photoreceptor
development and function in Danio rerio (zebrafish)
Dryad
dataset
2020
Electrophysiology
zebrafish retina
cone photoreceptors
ERG
2020-07-21T00:00:00Z
2020-07-21T00:00:00Z
en
543268 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
We investigate mutations in trβ2, a splice variant of thrb, identifying
changes in function, structure, and behavior in larval and adult zebrafish
retinas. Two N-terminus CRISPR mutants were identified. The first is a
6BP+1 insertion deletion frameshift resulting in a truncated protein. The
second is a 3BP in frame deletion with intact binding domains. ERG
recordings of isolated cone signals showed that the 6BP+1 mutants did not
respond to red wavelengths of light while the 3BP mutants did respond.
6BP+1 mutants lacked optomotor and optokinetic responses to red/black and
green/black contrasts. Both larval and adult 6BP+1 mutants exhibit a loss
of red-cone contribution to the ERG and an increase in UV-cone
contribution. Transgenic reporters show loss of cone trβ2 activation in
the 6BP+1 mutant but increase in the density of cones with active blue,
green, and UV opsin genes. Antibody reactivity for red-cone LWS1 and LWS2
opsin was absent in the 6BP+1 mutant, as was reactivity for arrestin3a.
Our results confirm a critical role for trβ2 in long-wavelength cone
development.
ERG Recording Raw Data Isolated amplitudes from cone photoreceptors were
collected through ERG electrode recordings. These datasets include the
information for the stimulus flash including irradiance and wavelength.
Responses are shown as the raw cone PIII amplitude response and the
normalized response. Isolated zebrafish eyes were stimulated with each
wavelength at varied irradiance levels. This stimulus information is then
listed along with the corresponding response on the same line of the
spreadsheet. The protocol runs through 70 stimuli per fish eye. Recordings
are separated into datasets by genotype (6BP+1/3BP/WT) and developmental
stage (larval/adult). There are only 6BP+1 and WT adults as 3BP mutants
did not survive to adulthood.
These datasets provide the irradiance, wavelength, and respective
amplitude response for 70 stimulus points per fish eye. There are 9
wavelengths: 650, 610, 570, 530, 490, 450, 410, 370, and 330. Repetition
of wavelength stimuli allows for log step increases in irradiance level
within each wavelength. Amplitude responses and their corresponding
stimulus wavelength and irradiance level can be used for modeling the
physiological properties of the cones. We used Origin Lab for analysis,
but other programs can use this data easily. Normalized amplitudes are
provided to control for non-biological variation between recordings. There
are no missing values.