10.5061/DRYAD.MKKWH70W8
Immler, Simone
0000-0003-1234-935X
University of East Anglia
Chen, Hwei-yen
University of Copenhagen
Bublys, Kasparas
Uppsala University
Jolly, Cecile
Uppsala University
Marcu, Daniel
0000-0002-2151-5850
University of East Angla
Data from: Trade-off between somatic and germline repair in a vertebrate
supports the expensive germ line hypothesis
Dryad
dataset
2020
Danio rerio
TUNEL assay
Male reproductive fitness
European Research Council
https://ror.org/0472cxd90
HapSelA336633
Knut and Alice Wallenberg Foundation
https://ror.org/004hzzk67
Wallenberg Academy Fellowsip Immler
International Human Frontier Science Program Organization
https://ror.org/02ebx7v45
RGP0025/2015
2020-03-26T00:00:00Z
2020-03-26T00:00:00Z
en
2863360 bytes
2
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The disposable soma theory is a central tenet of the biology of aging
where germline immortality comes at the cost of an aging soma [T. B. L.
Kirkwood, Nature 270, 301–304 (1977); T. B. L. Kirkwood, Proc. R. Soc.
Lond. B Biol. Sci. 205, 531–546 (1979); T. B. L. Kirkwood, S. N. Austad,
Nature 408, 233–238 (2000)]. Limited resources and a possible trade-off
between the repair and maintenance of the germ cells and growth and
maintenance of the soma may explain the deterioration of the soma over
time. Here we show that germline removal allows accelerated somatic
healing under stress. We tested “the expensive germ line” hypothesis by
generating germline-free zebrafish Danio rerio and testing the effect of
the presence and absence of the germ line on somatic repair under benign
and stressful conditions. We exposed male fish to sublethal low-dose
ionizing radiation, a genotoxic stress affecting the soma and the germ
line, and tested how fast the soma recovered following partial fin
ablation. We found that somatic recovery from ablation occurred
substantially faster in irradiated germline-free fish than in the control
germline-carrying fish where somatic recovery was stunned. The germ line
did show signs of postirradiation recovery in germline-carrying fish in
several traits related to offspring number and fitness. These results
support the theoretical conjecture that germline maintenance is costly and
directly trades off with somatic maintenance.
All the methods can be found in the main article and the supplementary
information.