10.5061/DRYAD.MH981
Parducci, Laura
Uppsala University
Väliranta, Minna
University of Helsinki
Salonen, J. Sakari
University of Helsinki
Ronkainen, Tiina
University of Helsinki
Matetovici, Irina
Babeș-Bolyai University
Fontana, Sonia L.
Queen's University Belfast
Eskola, Tiina
University of Oulu
Sarala, Pertti
Geological Survey of Finland
Suyama, Yoshihisa
Tohoku University
Data from: Proxy comparison in ancient peat sediments: pollen, macrofossil
and plant DNA
Dryad
dataset
2015
Alnus
Pinus
Nymphaea
Sphagnum
Salix
Genetics
Ulmus
Plant science
Sorbus
Betula
6000-52000 yr BP
Picea
2015-11-05T00:00:00Z
2015-11-05T00:00:00Z
en
https://doi.org/10.1098/rstb.2013.0382
47320 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
We compared DNA, pollen and macrofossil data obtained from Weichselian
interstadial and Holocene (maximum age 8400 cal yr BP) peat sediments from
northern Europe and used them to reconstruct contemporary floristic
compositions at two sites. The majority of the samples provided plant DNA
sequences of good quality with success amplification rates depending on
age. DNA and sequencing analysis provided five plant taxa from the older
site and nine taxa from the younger site, corresponding to 7% and 15% of
the total number of taxa identified by the three proxies together. At both
sites, pollen analysis detected the largest (54) and DNA the lowest (10)
number of taxa, but five of the DNA taxa were not detected by pollen and
macrofossils. The finding of a larger overlap between DNA and pollen than
between DNA and macrofossils proxies seems to go against our previous
suggestion based on lacustrine sediments that DNA originates principally
from plant tissues and less from pollen. At both sites, we also detected
Quercus spp. DNA, but few pollen grains were found in the record, and
these are normally interpreted as long-distance dispersal. We confirm that
in palaeoecological investigations, sedimentaryDNA analysis is less
comprehensive than classical morphological analysis, but is a
complementary and important tool to obtain a more complete picture of past
flora.
trnL sequencesThe file include trnL sequences obtained using
Sanger/cloning technique at Uppsala University from sedimentary (bulk) DNA
samples from peat layers located on in Northern Finland (NEF)and in
Northern Russia (NER)
Seida North-Eastern European Russia
Kaarreoja Finnish Lapland