10.5061/DRYAD.MD310
Li, Na
Shanxi Agricultural University
Wei, Jia-Ning
Zoological Society of London
Schuman, Meredith C.
Max Planck Institute for Chemical Ecology
German Center for Integrative Biodiversity Research
Li, Shuang
Shanxi Agricultural University
Ma, Rui-Yan
Shanxi Agricultural University
Ge, Jin
Zoological Society of London
Data from: Manipulating two olfactory cues causes a biological control
beetle to shift to non-target plant species
Dryad
dataset
2018
host plant preference
plant volatiles
Host Shift
(Z)-3-hexenol
non-target effect
Agasicles hygrophila
attraction and repulsion
2018-03-29T00:00:00Z
2018-03-29T00:00:00Z
en
https://doi.org/10.1111/1365-2745.12778
127810 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Olfactory cues can determine the host preferences of herbivorous insects,
but their role in host shifting is unclear. Host specificity and the
potential for host shifts are important criteria for screening and
post-release evaluation of biological control agents for invasive plants.
However, the role of olfactory cues in mediating host shifts in biological
control agents is not well understood. To investigate the role of
olfactory cues in host selection of a reportedly monophagous flea beetle
(Agasicles hygrophila), an important biocontrol agent for invasive
alligator weed (Alternanthera philoxeroides), we extracted and analysed
the volatiles produced by the host-plant A. philoxeroides and the non-host
plants Alternanthera sessilis, Beta vulgaris and Amaranthus mangostanus.
Moreover, we used electrophysiological techniques, behavioural bioassays
and field trials to test the antennal responses and behavioural
preferences of A. hygrophila to combinations of different plant volatiles
and treatments, and pure compounds in different dosages and combinations.
We show that A. hygrophila female beetles indeed use olfactory cues to
select plants for feeding and oviposition and that the survivorship of
larvae on the second preferred non-host plant A. sessilis, a close
relative of the first preferred host plant A. philoxeroides, was over 75%
in a field trail. Although female beetles responded to many volatile
compounds from host and non-host plants, (E)-4,8-dimethyl-1,3,7-nonatriene
(DMNT) positively encouraged the beetle's feeding and oviposition
preferences, whereas (Z)-3-hexenol displayed repellent effect. Remarkably,
complementation assays with (Z)-3-hexenol on host plant or DMNT on
non-host plants significantly shifted A. hygrophila host preferences to
non-host plants and resulted in oviposition and egg hatching on the
non-host plant A. sessilis in field trials. Synthesis. We demonstrate an
olfactory mechanism by which a specialized herbivorous beetle uses the
ratio of two common plant volatiles, (E)-4,8-dimethyl-1,3,7-nonatriene and
(Z)-3-hexenol, to discriminate between its host and non-host plants in
nature. This study highlights an important mechanism by which olfactory
cues could lead to undesired host range expansion in biocontrol agent,
thus representing an important warning of the potential for a host shift
and development of invasiveness in a common biocontrol agent, the flea
beetle.
Data of the study on Agasicles hygrophila flea beetleThis dataset is
deposited by authors of manuscript entitle "Manipulating two
olfactory cues causes a biological control beetle to shift to non-target
plant species", which would appear in Journal of Ecology. The dataset
includes all raw data of table 1-3, figures 1-4, supplementary Figures
S1-S3, and Supplementary Tables S1-S6 are included.Data.xlsx
China