10.5061/DRYAD.MB004D1
Rawls, John
Duke University
Ye, Lihua
Duke University
Mueller, Olaf
Duke University
Bagwell, Jennifer
Duke University
Bagnat, Michel
Duke University
Liddle, Rodger
Duke University
Data from: High fat diet induces microbiota-dependent silencing of
enteroendocrine cells
Dryad
dataset
2019
2019-12-04T00:00:00Z
2019-12-04T00:00:00Z
en
https://doi.org/10.7554/eLife.48479
1366734691 bytes
4
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Enteroendocrine cells (EECs) are specialized sensory cells in the
intestinal epithelium that sense and transduce nutrient information.
Consumption of dietary fat contributes to metabolic disorders, but EEC
adaptations to high fat feeding were unknown. Here, we established a new
experimental system to directly investigate EEC activity in vivo using a
zebrafish reporter of EEC calcium signaling. Our results reveal
that high fat feeding alters EEC morphology and converts them into a
nutrient insensitive state that is coupled to endoplasmic reticulum (ER)
stress. We called this novel adaptation 'EEC silencing'.
Gnotobiotic studies revealed that germ-free zebrafish are resistant
to high fat diet induced EEC silencing. High fat feeding altered
gut microbiota composition including enrichment of Acinetobacter species,
and we identified an Acinetobacter strain sufficient to induce
EEC silencing. These results establish a new mechanism by which
dietary fat and gut microbiota modulate EEC nutrient sensing and
signaling.
Source data for "High fat diet induces microbiota-dependent silencing
of enteroendocrine cells" Source data for Fig1-9 and Fig2-figure
supplement 1 Source data.zip