10.5061/DRYAD.M7Q4C
Echodu, Richard
Gulu University
Sistrom, Mark J.
Yale University
Bateta, Rosemary
Kenya Agricultural Research Institute
Murilla, Grace
Kenya Agricultural Research Institute
Okedi, Loyce
National Agricultural Research Organisation
Aksoy, Serap
Department of Epidemiology of Microbial Diseases, Yale School of Public
Health, New Haven, Connecticut, United States of America
Enyioha, Chineme
Department of Epidemiology of Microbial Diseases, Yale School of Public
Health, New Haven, Connecticut, United States of America
Enyaru, John
Makerere University
Opiyo, Elizabeth
Gulu University
Gibson, Wendy
University of Bristol
Adalgisa, Caccone
Sistrom, Mark
Yale University
Caccone, Adalgisa
Yale University
Data from: Genetic diversity and population structure of Trypanosoma
brucei in Uganda: implications for the epidemiology of sleeping sickness
and Nagana
Dryad
dataset
2015
Trypanosoma brucei
2015-10-28T00:00:00Z
2015-10-28T00:00:00Z
en
https://doi.org/10.1371/journal.pntd.0003353
7139 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Background: While Human African Trypanosomiasis (HAT) is in decline on the
continent of Africa, the disease still remains a major health problem in
Uganda. There are recurrent sporadic outbreaks in the traditionally
endemic areas in south-east Uganda, and continued spread to new unaffected
areas in central Uganda. We evaluated the evolutionary dynamics
underpinning the origin of new foci and the impact of host species on
parasite genetic diversity in Uganda. We genotyped 269 Trypanosoma brucei
isolates collected from different regions in Uganda and southwestern Kenya
at 17 microsatellite loci, and checked for the presence of the SRA gene
that confers human infectivity to T. b. rhodesiense. Results: Both
Bayesian clustering methods and Discriminant Analysis of Principal
Components partition Trypanosoma brucei isolates obtained from Uganda and
southwestern Kenya into three distinct genetic clusters. Clusters 1 and 3
include isolates from central and southern Uganda, while cluster 2
contains mostly isolates from southwestern Kenya. These three clusters are
not sorted by subspecies designation (T. b. brucei vs T. b. rhodesiense),
host or date of collection. The analyses also show evidence of genetic
admixture among the three genetic clusters and long-range dispersal,
suggesting recent and possibly on-going gene flow between them.
Conclusions: Our results show that the expansion of the disease to the new
foci in central Uganda occurred from the northward spread of T. b.
rhodesiense (Tbr). They also confirm the emergence of the human infective
strains (Tbr) from non-infective T. b. brucei (Tbb) strains of different
genetic backgrounds, and the importance of cattle as Tbr reservoir, as
confounders that shape the epidemiology of sleeping sickness in the
region.
alltrypsmicrosatellite data
Tanzania
Uganda