10.5061/DRYAD.M2V21
Thomé, Maria Tereza C.
Sao Paulo State University
Zamudio, Kelly R.
Cornell University
Haddad, Célio F. B.
Sao Paulo State University
Alexandrino, João
Federal University of Sao Paulo
Data from: Barriers, rather than refugia, underlie the origin of diversity
in toads endemic to the Brazilian Atlantic Forest
Dryad
dataset
2014
Rhinella crucifer group
habitat fragmentation
2014-11-06T17:05:55Z
2014-11-06T17:05:55Z
en
https://doi.org/10.1111/mec.12986
1608718 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
In this study, we investigated the relative contribution of geographic
barriers and Pleistocene refuges in the diversification of the Rhinella
crucifer species complex, a group of endemic toads with a widespread
distribution in the Brazilian Atlantic Forest (AF). We used intensive
sampling and multilocus DNA sequence data to compare nucleotide diversity
between refuge and nonrefuge areas, investigate regional demographic
patterns, estimate demographic parameters related to genetic breaks and
test refuge versus barrier scenarios of diversification using approximate
Bayesian computation. We did not find higher levels of genetic diversity
in putative refuge areas, either at regional or biome scale. Rather, the
demographic history of the species complex supports regional differences
with moderate population growth in the north and central regions and
stability in southern AF. Genetic breaks were dated to the
Plio–Pleistocene; however, our analyses rejected the role of refuges in
creating a northern and central divergence, supporting a recent
colonization scenario at a smaller scale within the central AF. Overall,
our data rule out massive climatically driven fragmentation and
large-scale recolonization events for populations across the biome. We
confirmed the importance of geographic barriers in creating main
divergences and underscored the importance of searching for cryptic
discontinuities in the landscape. Comparison of our results with those of
other AF taxa indicates organismal specific responses to moderate shifts
in habitat and that multiple refuges may constitute a more realistic model
for diversification of Atlantic Forest biota.
tableS1Voucher numbers, locality, coordinates, region, and genbank
assession numbers for individuals used in this studyND2alignment for the
NADH dehydrogenase subunit 2 fragmentcrystallinalignment for the
beta-crystallin fragmentalphapolypeptidealignment for the intron 1 of the
A alpha polypeptide fragmentABC_NxCperl script for the ABC analysis with
regions N and CABCwithinCperl script for the ABC analysis within region C