10.5061/DRYAD.KH7F2
Narum, Shawn R.
Columbia River Inter-Tribal Fish Commission
University of Idaho
Campbell, Nathan R.
Columbia River Inter-Tribal Fish Commission
Meyer, Kevin A.
Idaho Department of Fish and Game
Miller, Michael R.
University of Oregon
Hardy, Ronald W.
University of Idaho
Data from: Thermal adaptation and acclimation of ectotherms from differing
aquatic climates
Dryad
dataset
2013
Oncorhynchus mykiss gairdneri
2013-01-09T19:40:57Z
2013-01-09T19:40:57Z
en
https://doi.org/10.1111/mec.12240
7205773 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
To elucidate the mechanisms of thermal adaptation and acclimation in
ectothermic aquatic organisms from differing climates, we used a
common-garden experiment for thermal stress to investigate the heat shock
response of redband trout (Oncorhynchus mykiss gairdneri) from desert and
montane populations. Evidence for adaptation was observed as expression of
heat shock genes in fish from the desert population was more similar to
control (unstressed) fish and significantly different (P ≤ 0.05) from
those from the montane population, while F1 crosses were intermediate.
High induction of heat shock proteins (Hsps) in the montane strain
appeared to improve short-term survival during first exposure to high
water temperatures, but high physiological costs of Hsp production may
have led to lower long-term survival. In contrast, the desert strain had
significantly lower heat shock response than the montane fish and F1
crosses, suggesting that these desert fish have evolved alternative
mechanisms to deal with thermal stress that provide better balance of
physiological costs. Genomewide tests of greater than 10 000 SNPs found
multiple SNPs that were significantly associated with survival under
thermal stress, including Hsp47 which consistently appeared as a strong
candidate gene for adaption to desert climates. Candidate SNPs identified
in this study are prime targets to screen more broadly across this
species' range to predict the potential for adaptation under
scenarios of climate change. These results demonstrate that aquatic
species can evolve adaptive responses to thermal stress and provide
insight for understanding how climate change may impact ectotherms.
Heat_Shock_Gene_ExpressionNormalized gene expression values for six heat
shock genes for liver tissues from heat stressed strains of O. mykiss
gairderniSNP_Genotypes_PLINKinput.pedSNP genotypes from survivors and
mortalities of O. mykiss gairdneri under thermal stress.List of SNPsThis
file includes a list of all 10,497 SNPs along with their RAD tag IDs,
allele sequences, and SNP position. See ReadMe tab for further
details.Narum et al. SNP list.xlsx