10.5061/DRYAD.K0N0676
Karisto, Petteri
Swiss Federal Institute of Technology in Zurich
Dora, Susanne
Swiss Federal Institute of Technology in Zurich
Mikaberidze, Alexey
Swiss Federal Institute of Technology in Zurich
Data from: Measurement of infection efficiency of a major wheat pathogen
using time-resolved imaging of disease progress
Dryad
dataset
2019
plant pathology
Zymoseptoria tritici
Triticum aestivum
2019-08-15T00:00:00Z
en
https://doi.org/10.1111/ppa.12932
307043 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Infection efficiency is a key epidemiological parameter that determines
the proportion of pathogen spores able to infect and cause lesions once
they have landed on a susceptible plant tissue. In this study, we present
an improved method to measure infection efficiency of Zymoseptoria tritici
using a replicated greenhouse experiment. Z. tritici is a fungal pathogen
that infects wheat leaves and causes Septoria tritici blotch (STB), a
major disease of wheat worldwide. We devised an original experimental
setup, where we (i) attached living wheat leaves to metal plates allowing
for timeāresolved imaging of disease progress in planta. Since lesions
were continuously appearing, expanding and merging during the period of up
to three weeks, daily measurements were necessary for accurate counting of
lesions. We also (ii) used reference membranes to characterize the density
and the spatial distribution of inoculated spores on leaf surfaces. In
this way, we captured the relationship between the number of lesions and
the number of viable spores deposited on the leaves and estimated the
infection efficiency of about 4% from the slope of this relationship. Our
study provides a proof of principle for an accurate and reliable
measurement of infection efficiency of Z. tritici. The method opens
opportunities for determining the genetic basis of the component of
quantitative resistance that suppresses infection efficiency. This
knowledge would improve breeding for quantitative resistance against STB,
a control measure considered more durable than deployment of major
resistance genes.
Reference_membrane_colony_density_first_experimentFirst replicate of the
Infection efficiency experiment. The data was collected from the reference
membranes used to measure viable spore densities during the inoculation
spraying. The first column, "treatment", contains numbers giving
the spore concentration in the inoculated spore suspension, spores/ml. The
second column, "position" refers to position of the membrane on
the infection plate so that number 1 was at one end, number 2 next to it
and so on, from 1 to 5. The third column, "number" identifies
the counting square used to sample the colony density, arbitrary numbering
of up to two squares in the first and up to three squares in the second
experiment. The fourth column, "colony", gives the number of
colonies counted in the square. The fifth column, "percm", gives
the density of colonies within a square, colonies/cm^2. The sixth column,
"size", gives the size of a square, always "1", except
for highest concentration treatment of the second experiment, cm^2. Tab
delimited .txt
file.Karisto_Infection_Efficiency_Zymoseptoria_Reference_membrane_colony_density_first_experiment_2018.txtReference_membrane_colony_density_second_experimentSecond replicate of the Infection efficiency experiment. The data was collected from the reference membranes used to measure viable spore densities during the inoculation spraying. The first column, "treatment", contains numbers giving the spore concentration in the inoculated spore suspension, spores/ml. The second column, "position" refers to position of the membrane on the infection plate so that number 1 was at one end, number 2 next to it and so on, from 1 to 5. The third column, "number" identifies the counting square used to sample the colony density, arbitrary numbering of up to two squares in the first and up to three squares in the second experiment. The fourth column, "colony", gives the number of colonies counted in the square. The fifth column, "percm", gives the density of colonies within a square, colonies/cm^2. The sixth column, "size", gives the size of a square, always "1", except for highest concentration treatment of the second experiment, cm^2. Tab delimited .txt file.Karisto_Infection_Efficiency_Zymoseptoria_Reference_membrane_colony_density_second_experiment_2018.txtLesion_appearance_data_first_experimentLesion appearance on each leaf in the first replicate. The data was collected from daily photos by recording newly appeared lesions for every day. The first column, "treatment", contains numbers giving the spore concentration in the inoculated spore suspension, spores/ml. The second column, "density" gives the estimated vaible spore density calculated based on the reference membranes on two sides of the leaf set, spore/cm^2. The third column, "timepoint", gives the timepoint of the recording, days after inoculation. The fourth column, "leafset", identifies four leaf sets belonging to each treatment, from 1 to 4. The fifth column, "leafnumber", identifies each leaf in a leaf set, up to 8 leaves per set. The sixth column, "lesion", gives number of observed separate lesions on the image of the leaf at that date. The seventh column, "lesionappear", gives number of newly appearing lesions since the previous time point. The eighth column, "lesiontot", gives total number of appeared single lesions by that date, based on sum of appearance data of each timepoint. The ninth column, "leafsize", gives size of each leaf estimated from photos of the leaf sets taken on the day of inoculation, cm^2. The tenth column, "colony", gives estimated number of spores that landed on that leaf during the inoculation. The eleventh column, "leaflesion", gives density of all lesions appeared on the leaf (column "lesiontot"), lesions/cm^2. Tab delimited .txt file.Karisto_Infection_Efficiency_Zymoseptoria_Lesion_appearance_data_first_experiment_2018.txtLesion_appearance_data_second_experimentLesion appearance on each leaf in the second replicate. The data was collected from daily photos by recording newly appeared lesions for every day. The first column, "treatment", contains numbers giving the spore concentration in the inoculated spore suspension, spores/ml. The second column, "density" gives the estimated vaible spore density calculated based on the reference membranes on two sides of the leaf set, spore/cm^2. The third column, "timepoint", gives the timepoint of the recording, days after inoculation. The fourth column, "leafset", identifies four leaf sets belonging to each treatment, from 1 to 4. The fifth column, "leafnumber", identifies each leaf in a leaf set, up to 8 leaves per set. The sixth column, "lesion", gives number of observed separate lesions on the image of the leaf at that date. The seventh column, "lesionappear", gives number of newly appearing lesions since the previous time point. The eighth column, "lesiontot", gives total number of appeared single lesions by that date, based on sum of appearance data of each timepoint. The ninth column, "leafsize", gives size of each leaf estimated from photos of the leaf sets taken on the day of inoculation, cm^2. The tenth column, "colony", gives estimated number of spores that landed on that leaf during the inoculation. The eleventh column, "leaflesion", gives density of all lesions appeared on the leaf (column "lesiontot"), lesions/cm^2. Tab delimited .txt file.Karisto_Infection_Efficiency_Zymoseptoria_Lesion_appearance_data_second_experiment_2018.txt