10.5061/DRYAD.JM65K
Keirle, Matthew R
Avis, Peter G
Feldheim, Kevin A
Hemmes, Don E
Mueller, Gregory M
Data from: Investigating the allelic evolution of an imperfect
microsatellite locus in the Hawaiian mushroom Rhodocollybia laulaha
Dryad
dataset
2011
Gene regulation
Gene transmission
Rhodocollybia laulaha
gene action
2011-08-15T15:27:30Z
2011-08-15T15:27:30Z
en
https://doi.org/10.1093/jhered/esr099
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The evolutionary mechanisms that give rise to microsatellite alleles
remain poorly understood in general and are especially understudied for
fungal microsatellite loci. The unusual G28 microsatellite locus was
developed from the Hawaiian mushroom Rhodocollybia laulaha. Here we employ
a novel approach to test for allele size homoplasy and examine competing
mechanistic models of microsatellite evolution in the context of
biogeographic expectations for this locus based on Hawaiian geologic
history. Seven G28 alleles have been identified from a sampling of 153
individuals. The G28 locus is comprised of a tri-nucleotide, imperfect
motif which permits examination of the relationships between alleles and
allows for detection of potential size homoplasy within the repetitive
element. Alignment of G28 allele sequence data across multiple unrelated
individuals suggests that alleles of like size are homologous within
Hawaii. A variety of gap coding methods are explored in the inference of
allele evolution. Length differences between alleles appear to be the
result of polymerase slippage at multiple positions in the repetitive
element suggesting an intricate process of allelic evolution which is not
necessarily stepwise. Complex migration scenarios must be invoked to
explain the current geographic distribution of alleles if their evolution
was in fact sequential (from longest to shortest or from shortest to
longest) as predicted by the “progression rule”.
Keirle et al., (SUPPLEMENTARY DOCUMENTS)
Hawaii