10.5061/DRYAD.JM5S0
Lang, Zhihong
University of Wisconsin-Madison
Wills, David M.
University of Wisconsin-Madison
Lemmon, Zachary H.
University of Wisconsin-Madison
Shannon, Laura M.
University of Wisconsin-Madison
Bukowski, Robert
University of Wisconsin-Madison
Wu, Yongrui
University of Wisconsin-Madison
Messing, Joachim
University of Wisconsin-Madison
Doebley, John F.
University of Wisconsin-Madison
Data from: Defining the role of Prolamin-box binding factor1 gene during
maize domestication
Dryad
dataset
2014
Teosinte
Gene mapping
regulation and transmission
RNAseq
gene action
2014-03-18T19:18:43Z
2014-03-18T19:18:43Z
en
https://doi.org/10.1093/jhered/esu019
26359 bytes
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CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
The prolamin-box binding factor1 (pbf1) gene encodes a transcription
factor that controls the expression of seed storage protein (zein) genes
in maize. Prior studies show that pbf1 underwent selection during maize
domestication although how it affected trait change during domestication
is unknown. To assay how pbf1 affects phenotypic differences between maize
and teosinte, we compared nearly isogenic lines (NILs) that differ for a
maize versus teosinte allele of pbf1. Kernel weight for the teosinte NIL
(162mg) is slightly but significantly greater than that for the maize NIL
(156mg). RNAseq data for developing kernels show that the teosinte allele
of pbf1 is expressed at about twice the level of the maize allele.
However, RNA and protein assays showed no difference in zein profile
between the two NILs. The lower expression for the maize pbf1 allele
suggests that selection may have favored this change; however, how reduced
pbf1 expression alters phenotype remains unknown. One possibility is that
pbf1 regulates genes other than zeins and thereby is a domestication
trait. The observed drop in seed weight associated with the maize allele
of pbf1 is counterintuitive but could represent a negative pleiotropic
effect of selection on some other aspect of kernel composition.
pdf all trait datadata for kernel traits.