10.5061/DRYAD.J3CK6
Wright, Sara J.
Washington University in St. Louis
Zhou, Daniel Cui
Washington University in St. Louis
Kuhle, Amy
Quincy University
Olsen, Kenneth M.
Washington University in St. Louis
Data from: Continent-wide climatic variation drives local adaptation in
North American white clover
Dryad
dataset
2017
Climatic Adaptation
environmental distance
adaptive polymorphism
cyanogenesis
National Science Foundation
https://ror.org/021nxhr62
DEB-0845497, IOS-1557770, DGE-1143954, DEB-1601641
2017-07-28T13:11:30Z
2017-07-28T13:11:30Z
en
https://doi.org/10.1093/jhered/esx060
2380692668 bytes
1
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Climate-associated clines in adaptive polymorphisms are commonly cited as
evidence of local adaptation within species. However, the contribution of
the clinally varying trait to overall fitness is often unknown. To address
this question, we examined survival, vegetative growth and reproductive
output in a central US common garden experiment using 161 genotypes of
white clover (Trifolium repens L.) originating from 15 locations across
North America. White clover is polymorphic for cyanogenesis (hydrogen
cyanide release upon tissue damage), a chemical defense against generalist
herbivores, and climate-associated cyanogenesis clines have evolved
repeatedly across the species range. Over a 12 month experiment, we
observed striking correlations between population of origin and plant
performance in the common garden, with climatic distance from the common
garden site predicting fitness more accurately than geographic distance.
Assessments of herbivore leaf damage over the 2015 growing season
indicated marginally lower herbivory on cyanogenic plants; however, this
effect did not result in increased fitness in the common garden location.
Linear mixed modeling suggested that while cyanogenesis variation had
little predictive value for vegetative growth, it is as important as
climatic variation for predicting reproductive output in the central US.
Together, our findings suggest that knowledge of climate similarity, as
well as knowledge of locally favored adaptive traits, will help to inform
transplantation strategies for restoration ecology and other conservation
efforts in the face of climate change.
White clover pictures (April)This file contains pictures of 483 white
clover plants taken at 1 of 4 time points over the course of a 12 month
common garden experiment in St. Louis, MO. The pictures have been cropped
to remove adjacent plants, and red circles have been added to the pictures
using ImageJ software. These circles cover pennies that were present for
scale in the original images. For this publication, the pictures were
analyzed with Easy Leaf Area software (Easlon & Bloom
2014).1of4-April.zipWhite clover pictures (May)This file contains pictures
of 483 white clover plants taken at 1 of 4 time points over the course of
a 12 month common garden experiment in St. Louis, MO. The pictures have
been cropped to remove adjacent plants, and red circles have been added to
the pictures using ImageJ software. These circles cover pennies that were
present for scale in the original images. For this publication, the
pictures were analyzed with Easy Leaf Area software (Easlon &
Bloom 2014).2of4-May.zipWhite clover pictures (October)This file contains
pictures of 483 white clover plants taken at 1 of 4 time points over the
course of a 12 month common garden experiment in St. Louis, MO. The
pictures have been cropped to remove adjacent plants, and red circles have
been added to the pictures using ImageJ software. These circles cover
pennies that were present for scale in the original images. For this
publication, the pictures were analyzed with Easy Leaf Area software
(Easlon & Bloom 2014).3of4-October.zipWhite clover pictures
(March)This file contains pictures of 483 white clover plants taken at 1
of 4 time points over the course of a 12 month common garden experiment in
St. Louis, MO. The pictures have been cropped to remove adjacent plants,
and red circles have been added to the pictures using ImageJ software.
These circles cover pennies that were present for scale in the original
images. For this publication, the pictures were analyzed with Easy Leaf
Area software (Easlon & Bloom 2014).4of4-March.zipVCF file with
SNP dataThis VCF file contains SNPs generated using genotyping by
sequencing (GBS), which were used for population genomics analyses in this
paper. The file contains data from additional white clover samples not
used in the study. See the two separate files for more information on the
individual samples.all.mergedSNPs.mergedTaxa.vcf.gzSample Key for VCF
fileThis file contains information on the individual samples included in
the VCF file.white_clover_olsen_key.txt
North America